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Table 1 Methanogen 16S rRNA gene clones from the 25th anaerobic fungal subculture

From: Discovery of a novel rumen methanogen in the anaerobic fungal culture and its distribution in the rumen as revealed by real-time PCR

16S rRNA phylotype No. of clones Size (bp) GenBank accession number Nearest valid taxon Sequence identity (%)
LGM-AF01 51 1260 DQ985539 Methanobrevibacter sp. Z8 97.8
LGM-AF02 1 1260 DQ985538 Methanobrevibacter sp. Z8 97.6
LGM-AF03 1 1260 DQ985541 Methanobrevibacter sp. 30Y 97.5
LGM-AF04 2 1256 DQ985540 Candidatus Methanomethylophilus alvus Mx1201 93.0
LGM-AF05 2 1260 DQ985542 Methanobrevibacter sp. Z8 97.7
LGM-AF06 1 1260 DQ985543 Methanobrevibacter sp. Z8 97.5
LGM-AF07 1 1260 DQ985544 Methanobrevibacter sp. Z8 97.6
LGM-AF08 2 1260 DQ985545 Methanobrevibacter sp. Z8 97.5
LGM-AF09 1 1260 DQ985546 Methanobrevibacter sp. Z8 97.6
LGM-AF10 1 1260 DQ985547 Methanobrevibacter sp. Z8 97.5
LGM-AF11 1 1260 DQ985548 Methanobrevibacter sp. Z8 97.5
LGM-AF12 1 1260 DQ985549 Methanobrevibacter sp. 30Y 97.7
LGM-AF13 1 1260 DQ985550 Methanobrevibacter sp. Z8 97.4
  1. A total of 66 clones were examined.