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Table 1 Methanogen 16S rRNA gene clones from the 25th anaerobic fungal subculture

From: Discovery of a novel rumen methanogen in the anaerobic fungal culture and its distribution in the rumen as revealed by real-time PCR

16S rRNA phylotype

No. of clones

Size (bp)

GenBank accession number

Nearest valid taxon

Sequence identity (%)

LGM-AF01

51

1260

DQ985539

Methanobrevibacter sp. Z8

97.8

LGM-AF02

1

1260

DQ985538

Methanobrevibacter sp. Z8

97.6

LGM-AF03

1

1260

DQ985541

Methanobrevibacter sp. 30Y

97.5

LGM-AF04

2

1256

DQ985540

Candidatus Methanomethylophilus alvus Mx1201

93.0

LGM-AF05

2

1260

DQ985542

Methanobrevibacter sp. Z8

97.7

LGM-AF06

1

1260

DQ985543

Methanobrevibacter sp. Z8

97.5

LGM-AF07

1

1260

DQ985544

Methanobrevibacter sp. Z8

97.6

LGM-AF08

2

1260

DQ985545

Methanobrevibacter sp. Z8

97.5

LGM-AF09

1

1260

DQ985546

Methanobrevibacter sp. Z8

97.6

LGM-AF10

1

1260

DQ985547

Methanobrevibacter sp. Z8

97.5

LGM-AF11

1

1260

DQ985548

Methanobrevibacter sp. Z8

97.5

LGM-AF12

1

1260

DQ985549

Methanobrevibacter sp. 30Y

97.7

LGM-AF13

1

1260

DQ985550

Methanobrevibacter sp. Z8

97.4

  1. A total of 66 clones were examined.