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Table 1 DNA purity and 16 s rRNA sequence reads by fecal collection method

From: Comparison of methods for fecal microbiome biospecimen collection

Methoda OD 260/280 (Mean ± SD)b Filtered sequence reads (Mean ± SD)d
Method 1: Card 1.86 ± 0.05 12,448 ± 8,761
Method 2: Room Temperature 1.81 ± 0.07 16,451 ± 12,004
Method 3: RNAlater 1.66 ± 0.14c 13,393 ± 5,909
Method 4: Frozen 1.80 ± 0.05 14,467 ± 10,030
  1. a1: fecal occult blood test cards at room temperature for 3 days, 2: Eppendorf tubes at room temperature for 3 days, 3: Eppendorf tubes with RNAlater at room temperature for 3 days or 4: frozen at −80°C for 3 days.
  2. bAnova was used to test for overall differences across storage methods (p < 0.005).
  3. cBased on Anova results, we conducted Post Hoc TEST (LSD method) to make multiple comparisons, indicating that Method 3 resulted in lower OD 260/280 ratio (p < 0.05).
  4. dKruskal-Wallis was used to test for overall differences across storage methods (p = 0.84).