Figure 2

Spore germination of slow-germinating strains and of gerAA disruption mutant complemented with gerA sequences from slow-germinating strains. ab: Germination of MW3∆gerAA (x), the wild-type strains ATCC14580 (■), NVH 1032 (▲), NVH1112 (●) and NVH800 (♦) measured as reduction in absorbance (A₆₀₀) after addition of germinant (100 mM L-alanine). cd: Spore germination of the MW3∆gerAA (x), and MW3∆gerAA complemented with gerA from ATCC14580 (□ NVH1311), NVH1032 (∆ NVH1309), NVH1112 (○ NVH1321) and NVH800 (◊ NVH1322) measured as reduction in absorbance (A₆₀₀) after addition of germinant (100 mM L-alanine). The results represent the average (SD) of three independent spore batches. The type strain derivate MW3 (dotted line) has been included in Figure 3D for comparison.