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Figure 5 | BMC Microbiology

Figure 5

From: Development of loop-mediated isothermal amplification assay for specific and rapid detection of differential goat Pox virus and Sheep Pox virus

Figure 5

Specificity of LAMP for detection of different pathogenic nucleic acids using different primer sets. Approximately 100 ng of DNA or cDNA template from ten different sheep or goat pathogens was used in LAMP reaction. (a) GSPV primers amplification products. (b) GTPV primer amplification products. (c) SPPV primer amplification products. Agarose gel electrophoresis (2.5%) of LAMP products stained with ethidium bromide and visualized under a UV transilluminator. Lane 1: GTPV; Lane 2: SPPV; Lane 3: Orf virus; Lane 4: FMDV O/China99; Lane 5: M. ovipneumoniae; Lane 6: Chlamydia psittaci; Lane 7: L.interrogans; Lane 8: Toxoplasma gondii; Lane 9:Babesia sp; Lane 10: Theileria; C: no template control (NTC) and Lane M: 100 bp DNA Ladder Marker (TaKaRa, Dalian).

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BMC Microbiology

ISSN: 1471-2180

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