Table 3 Oligonucleotide primers used for PCR
Primer names | Oligo sequences (from 5′–3′) | Purpose | Reference |
|---|---|---|---|
BPSS2242-F1 | ACCGCGCGACCGATATGAACG | Forward primer for upstream fragment of SDO gene | This study |
BPSS2242-F2 | GGACTCCTTGCCGAACGGGC | Reverse primer for upstream fragment of SDO gene | This study |
BPSS2242-R1 | GCCCGTTCGGCAAGGAGTCC AACGTCGAGGCGAAGCTGCC | Forward primer for downstream fragment of SDO gene | This study |
BPSS2242-R2 | TCCCTTCGCGCTCGTGCAAC | Reverse primer for downstream fragment of SDO gene | This study |
OriT-F | CAGCCTCGCAGAGCAGGATTC | Forward primer for oriT | [50] |
OriT-R | TCCGCTGCATAACCCTGCTTC | Reverse primer for oriT | [50] |