A functional VipA-VipB interaction is required for the type VI secretion system activity of Vibrio cholerae O1 strain A1552

Table 1 Protein-protein interactions in the yeast two-hybrid assay

VipA mutants fused to the GAL4 activation domain of plasmid pGADT7 were co-transformed with VipB on the GAL4 DNA-binding domain pGBKT7 into the S. cerevisiae reporter strain Y187. Activation of the lacZ reporter from 4 independent experiments where duplicate transformants were tested on each occasion was determined and expressed as % mean β-galactosidase activity ± SEM relative to the activity of the wild-type protein. A Student’s 2-sided t-test was used to determine whether the differences observed were statistically significant (*, P < 0.05; ***, P < 0.001).

ISSN: 1471-2180