DNA-binding domain
|
Activation domain
|
Relative β-gal activity
|
---|
VipB
|
None
|
0.5 ± 0.1% ***
|
VipB
|
VipA
|
100.0 ± 5.8%
|
VipB
|
VipA Δ104-113
|
1.0 ± 0.2% ***
|
VipB
|
VipA D104A
|
92.7 ± 4.1%
|
VipB
|
VipA V106A
|
92.4 ± 3.4% *
|
VipB
|
VipA V110A
|
74.6 ± 3.4% ***
|
VipB
|
VipA D104A/V106A
|
64.1 ± 10.7% *
|
VipB
|
VipA V110A/L113A
|
1.1 ± 0.3% ***
|
VipB
|
VipA D104A/V106A/V110A
|
48.8 ± 2.0% ***
|
VipB
|
VipA D104A/V106A/V110A/L113A
|
1.0 ± 0.2% ***
|
- VipA mutants fused to the GAL4 activation domain of plasmid pGADT7 were co-transformed with VipB on the GAL4 DNA-binding domain pGBKT7 into the S. cerevisiae reporter strain Y187. Activation of the lacZ reporter from 4 independent experiments where duplicate transformants were tested on each occasion was determined and expressed as % mean β-galactosidase activity ± SEM relative to the activity of the wild-type protein. A Student’s 2-sided t-test was used to determine whether the differences observed were statistically significant (*, P < 0.05; ***, P < 0.001).