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Table 1 Protein-protein interactions in the yeast two-hybrid assay

From: A functional VipA-VipB interaction is required for the type VI secretion system activity of Vibrio cholerae O1 strain A1552

DNA-binding domain Activation domain Relative β-gal activity
VipB None 0.5 ± 0.1% ***
VipB VipA 100.0 ± 5.8%
VipB VipA Δ104-113 1.0 ± 0.2% ***
VipB VipA D104A 92.7 ± 4.1%
VipB VipA V106A 92.4 ± 3.4% *
VipB VipA V110A 74.6 ± 3.4% ***
VipB VipA D104A/V106A 64.1 ± 10.7% *
VipB VipA V110A/L113A 1.1 ± 0.3% ***
VipB VipA D104A/V106A/V110A 48.8 ± 2.0% ***
VipB VipA D104A/V106A/V110A/L113A 1.0 ± 0.2% ***
  1. VipA mutants fused to the GAL4 activation domain of plasmid pGADT7 were co-transformed with VipB on the GAL4 DNA-binding domain pGBKT7 into the S. cerevisiae reporter strain Y187. Activation of the lacZ reporter from 4 independent experiments where duplicate transformants were tested on each occasion was determined and expressed as % mean β-galactosidase activity ± SEM relative to the activity of the wild-type protein. A Student’s 2-sided t-test was used to determine whether the differences observed were statistically significant (*, P < 0.05; ***, P < 0.001).