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Table 4 List of primers used for qRT-PCR

From: Genetic analysis of the roles of agaA, agaI, and agaS genes in the N-acetyl-D-galactosamine and D-galactosamine catabolic pathways in Escherichia colistrains O157:H7 and C

Namea

Strainb

Sequence

5RTagaA

EDL933

CCGTTTCTCAGCACACCTTA

3RTagaA

EDL933

CCCAGCATCACTCGTACATT

5RTnagA

EDL933

TTACCTTTGCCACCCATCTG

3RTnagA

EDL933

GCAGGCCATCAGCGATAATA

5RTnagB

EDL933

ATCTGTTTATGGGCGGTGTAG

3RTnagB

EDL933

GAGTGTCATGAGTCAGGGTTT

5RTagaA

E. coli C

ACTTCACGCCGCAGAATAA

3RTagaA

E. coli C

GCTGAGAAACGGCAATCAAC

5RTagaR

E. coli C

ACGGTATGAACGTGGCTAATG

3RTagaR

E. coli C

CAGCCTGATCGCCGTAAA

5RTagaS

Both

ATCCGCTGCTGTTGATCTC

3RTagaS

Both

GGTGATAGCATTCCGGTACAA

5RTnagA

E. coli C

CCGTGGCTGAATCTGGTAAA

3RTnagA

E. coli C

ATGACGTCGGCGTTCTTAC

5RTnagB

E. coli C

ATCTGTTTATGGGCGGTGTAG

3RTnagB

E. coli C

GAGTGTCATGAGTCAGGGTTT

5RTgapA

Both

CGACCTGTTAGACGCTGATTAC

3RTgapA

Both

CGATCAGATGACCGTCTTTCAC

  1. a The primer names indicate the genes that are targeted for quantification of transcript. The number, 5 preceding the name of the gene indicate forward primers and the number, 3 preceding the name of the gene indicates reverse primers.
  2. b The strain name indicates the sequence used to design the primer was from that strain and when the same primer is used for both strains it is indicated as both.
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BMC Microbiology

ISSN: 1471-2180

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