Namea | Strainb | Sequence (5' to 3') |
---|---|---|
Primers for gene knockouts | ||
5agaA | Both | GGCGTTGATGTAATGGATGACGCGCCGGATGTACTCGACAATGGTGTAGGCTGGAGCTGCTTC |
3agaA | Both | CTGCCGCATCAACAGACAGCGTACTGCCCGCCAG CCACCATTATTCCGGGGATCCGTCGACC |
5nagA | Both | TAGCGGAACTGCCGCCAGAGATCGAACAACGTTCACTGAAAATGGTGTAGGCTGGAGCTGCTTC |
3nagA | Both | AGGATGATATGTGGACCGGCAGCGACGATGTCGCTGCTTTATTATTCCGGGGATCCGTCGACC |
5nagB | Both | AATCCGCCAACGGCTTACATTTTACTTATTGAGGTGAATAATGGTGTAGGCTGGAGCTGCTTC |
3nagB | Both | AAATATTGCCCTGAGCAAGGAGCCAGGGCAGGGATAACAAATTATTCCGGGGATCCGTCGACC |
5agaI | Both | TGTGCTCTCTATTGTTTGTTTCCGCATTCGGCATTTTGTAAATGGTGTAGGCTGGAGCTGCTTC |
3agaI | EDL933 | ATAAGTTAATTTAAACATTTTGAGCAATTTTTCATCTGGATTATTCCGGGGATCCGTCGACC |
3agaI | E. coli C | GGCGACCCGCGGTTTTTAACATCTCATGTTGCTGTGTTCTATTATTCCGGGGATCCGTCGACC |
5agaS | EDL933 | TGCGGATCATCCTGACCGGAGCCGGAACCTCGGCATTTATATGGTGTAGGCTGGAGCTGCTTC |
5agaS | E. coli C | CTGCGGATCATCCTGACCGGAGCCGGAACGTCGGCATTTATATGGTGTAGGCTGGAGCTGCTTC |
3agaS | Both | AGGATGATATGTGGACCGGCAGCGACGATGTCGCTGCTTTATTATTCCGGGGATCCGTCGACC |
5agaR | E. coli C | ACGCAGCGTTGCGAAAGCTGCCGTTGAGTTGATTCAGCCAATGGTGTAGGCTGGAGCTGCTTC |
3agaR | E. coli C | CTGACGCCGCGCTCCAGATCGATCGCATCTACACCAAGAAATTATTCCGGGGATCCGTCGACC |
Primers for PCR and sequencing for verification of gene knockouts | ||
FagaA | Both | ATGACACACGTTCTGCGCGCCAG |
RagaA | Both | TCAAAACGAAGCTAATTGACCCTG |
FnagA | Both | ATGTGGACCATCAGCTGTCTGC |
RnagA | Both | TTCTTTGATCAGCCCGCGTTCGA |
FnagB | EDL933 | TATCGCAAATTAAACGAGTGTCT |
RnagB | Both | GTTCAGTGAACGTTGTTCGATCTCT |
FnagB | E. coli C | TATCGCAAATTAAACGCGTGTCT |
RagaI | Both | TGACATTCGTTTGCCATCGACAGTAC |
FagaI | EDL933 | GACTTTGCTGCGCCAGGGGGCGAGT |
RagaI | E. coli C | TGAGCAAATTTTTCATCTGGTTAGG |
FagaS | Both | CATCCAGCAATCCTTTTGCTTC |
RagaS | EDL933 | TAGATCTCTTCCAGCGCGATATGTT |
RagaS | E. coli C | TAGATCTCTTCCAGCGCGATGTGTT |
FagaR | E. coli C | ATGAGTAATACCGACGCTTCAGGT |
RagaR | E. coli C | ACCAGAATCACTTCAACCCCAGCC |
Primers for cloning genes | ||
5nagAHindIII | Both | GCATAAGCTTACATTTTACTTATTGAGGTGAATAATGTATGCATTAACCCAGGGCCGGATC |
3nagASmaI | Both | GCATCCCGGGTTATTGAGTTACGACCTCGTTACCGTTAA |
5agaAHindIII | EDL933 | GCATAAGCTTCAGTAATCTGAACTGGAGAGGAAAATGTCCGGTCGAGGAAGGGATATGACA |
5agaAHindIII | E. coli C | GCATAAGCTTCAGTAATCTGAACTGGAGAGGAAAATGTCCGGTCGAGGAAGGAATATGACA |
3agaAPstI | Both | GCATCTGCAGTCAAAACGAAGCTAATTGACCCTGAATCC |
5agaIHindIII | E. coli C | GCATAAGCTTGTTCATCAGACTAAGGATTGAGTTATGGAACGAGGCACTGCGTCTGGTGG |
3agaISmaI | E. coli C | GCATCCCGGGTTAAGGTGTTAATTAAACAAATAAAGTTC |
5nagBHindIII | E. coli C | GCATAAGCTTACATTTTACTTATTGAGGTGAATA |
3nagBSmaI | E. coli C | GCATCCCGGGTTACAGACCTTTGATATTTTCTGC |
5agaSHindIII | EDL933 | GCATAAGCTTGTTCATCAGACTAAGGATTGAGTT |
3agaSPst1 | EDL933 | GCATCTGCAGTTATGCCTGCCACGGATGAATGATTACGC |
3agaYPst1 | EDL933 | GCATCTGCAGTTATGCTGAAATTCGAATTCGCTG |
5agaSDHindIII | E. coli C | TAGCATAAGCTTATGCCAGAAAATTACACCCCT |
3agaSDEcoR1 | E. coli C | TAGCATGAATTCTTACAAAATGCCGAATGCGGA |
5agaBDHindIII | E. coli C | GCATAAGCTTGTTCATCAGACTAAGGATTGAGTTATGACCAGTCCAAATATTCTCTTAAC |
3agaBDSmaI | E. coli C | GCATCCCGGGTTACAAAATGCCGAATGCGGAACAAACAA |