Colonization strategies of Pseudomonas fluorescens Pf0-1: activation of soil-specific genes important for diverse and specific environments

Table 1 Bacterial strains and plasmids

Strains, plasmids or primers	Genotype or description	Reference or source
E. coli
DH5αλ pir	φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 deoR λpir	[19]
P. fluorescens
Pf0-1	Wild type, Amp^r	[20]
PfΔdap	Pf0-1 ΔdapB	This Study
Pf0-1::pKNOCK Fr2	Pf0-1 with partial deletion of Fr2	This Study
Pf0-1::pKNOCK Fr4	Pf0-1 with partial deletion of Fr4	This Study
Pf0-1::pKNOCK Fr9	Pf0-1 with partial deletion of Fr9	This Study
Pf0-1::pKNOCK Fr10	Pf0-1 with partial deletion of Fr10	This Study
Pf0-1::pKNOCK Fr2pJB	Pf0-1 with partial deletion of Fr2 carrying pJB866	This Study
Pf0-1::pKNOCK Fr2pJB Fr2	Pf0-1 with partial deletion of Fr2 Complemented by pJB866::Fr2	This Study
Pf0-1::pKNOCK Fr10pJB	Pf0-1 with partial deletion of Fr10 carrying pJB866	This Study
Pf0-1::pKNOCK Fr10pJB Fr10	Pf0-1 with partial deletion of Fr10 Complemented by pJB866::Fr10	This Study
Plasmids
pGEM-T Easy	Ap^r; cloning vector for PCR products	Promega
pIVETdap	dapB’ cloned in SpeI site of pUIC3	[11]
pJB866	Broad-host-range vector, Tc^r	[21]
pRK2013	Helper plasmid, IncP Tra⁺ Mob⁺ ColE1, Km^r	[21]
pKNOCK-Km	pBSL63 derivative carrying RP4 oriT and R6K γ-ori, Km^r	[22]
pSR47s	Km^r; sacB-containing suicide vector (requiring R6K replication origin)	[23]

ISSN: 1471-2180