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Table 1 Bacterial strains and plasmids

From: Colonization strategies of Pseudomonas fluorescens Pf0-1: activation of soil-specific genes important for diverse and specific environments

Strains, plasmids or primers Genotype or description Reference or source
E. coli   
  DH5αλ pir φ80dlacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 deoR λpir [19]
P. fluorescens   
  Pf0-1 Wild type, Ampr [20]
  PfΔdap Pf0-1 ΔdapB This Study
  Pf0-1::pKNOCK Fr2 Pf0-1 with partial deletion of Fr2 This Study
  Pf0-1::pKNOCK Fr4 Pf0-1 with partial deletion of Fr4 This Study
  Pf0-1::pKNOCK Fr9 Pf0-1 with partial deletion of Fr9 This Study
  Pf0-1::pKNOCK Fr10 Pf0-1 with partial deletion of Fr10 This Study
  Pf0-1::pKNOCK Fr2pJB Pf0-1 with partial deletion of Fr2 carrying pJB866 This Study
  Pf0-1::pKNOCK Fr2pJB Fr2 Pf0-1 with partial deletion of Fr2 Complemented by pJB866::Fr2 This Study
  Pf0-1::pKNOCK Fr10pJB Pf0-1 with partial deletion of Fr10 carrying pJB866 This Study
  Pf0-1::pKNOCK Fr10pJB Fr10 Pf0-1 with partial deletion of Fr10 Complemented by pJB866::Fr10 This Study
Plasmids   
  pGEM-T Easy Apr; cloning vector for PCR products Promega
  pIVETdap dapB’ cloned in SpeI site of pUIC3 [11]
  pJB866 Broad-host-range vector, Tcr [21]
  pRK2013 Helper plasmid, IncP Tra+ Mob+ ColE1, Kmr [21]
  pKNOCK-Km pBSL63 derivative carrying RP4 oriT and R6K γ-ori, Kmr [22]
  pSR47s Kmr; sacB-containing suicide vector (requiring R6K replication origin) [23]