Figure 5

Chemokine and cytokine production of different mouse inbred strains after oral infection with Lmo-EGD-lux and Lmo-InlA-mur-lux. Female C3HeB/FeJ (A), A/J OlaHsd (B), BALB/cJ (C) and C57BL/6J mice (D) were orally infected with 5 × 10⁹ CFU Lmo-EGD-lux or Lmo-InlA-mur-lux. Blood samples were collected at 3 and 5 d.p.i. and cytokine and chemokine levels were determined using Luminex bead assays. 3d and 5d indicate Lmo-EGD-lux infected animals at 3 and 5 d.p.i., respectively; 3d mur and 5d mur indicate Lmo-InlA-mur-lux infected animals at these timepoints (n = 8). For each timepoint, chemokine and cytokine concentrations were determined in triplicate for each inbred mouse and L. monocytogenes strain. Data represent means ± SEM. (E-H) Comparison of chemokine and cytokine production across Lmo-InlA-mur-lux infected mice from the different inbred mouse strains at 5 d.p.i.. Shown are statistical significant differences of indicated cytokine and chemokine levels in the peripheral blood between groups of mice of the analysed inbred mouse strains. Data represent means ± SEM; *p < 0.05, non-parametric Mann–Whitney-U-test. One out of two representative experiments is shown (A-H).