Figure 4

Susceptibility of C3HeB/FeJ mice to orally acquired listeriosis correlates with severe necrotic lesions in liver and spleen. Photographs of haematoxylin and eosin stained sections of liver (A to D) and spleen (E to H) from C3HeB/FeJ mice and C57BL/6J mice at three and five days post oral infection with L. monocytogenes. There are multifocal to coalescing areas of hepatic and splenic necrosis accompanied by neutrophils, macrophages and lymphocytes (arrows). The lesions are substantially more extensive in C3HeB/FeJ mice, and increase in severity from day 3 to day 5 p.i. In 4G the splenic necrosis in the C3HeB/FeJ mice has expanded to entirely efface the normal splenic architecture, while in the C57BL/6J mice (4H) the lesion has progressed to a focal aggregate of macrophages with minimal necrosis. The images presented are representative of changes seen in both Lmo-InlA-mur-lux and Lmo-EGD-lux infected animals (A: EGD-lux; B: InlA-mur-lux; C: EGD-lux; D: EGD-lux; E: EGD-lux; F: InlA-mur-lux, G: EGD-lux; H: InlA-mur-lux).