Table 2 Primers used in this study
Primer | Sequence (5′ → 3′) |
|---|---|
FRT-leftF | AATCCATCTTGTTCAATCATGC |
FRT-rightR | AATTCGAGCTCGGGAAGATC |
FRT-T7F | AAATTAATACGACTCACTATAGG |
FRT-SP6R | TACGATTTAGGTGACACTATAG |
Inv-pUC118F | CAACGTCGTGACTGGGAAAAC |
Inv-pUC118R | TCATGGTCATAGCTGTTTCCTG |
TetR-FLP2F | CGATGGGTGGTTAACTCGAC |
TetR-FLP2R | ACAGGACGGGTGTGGTCG |
AtaAupstF | CGCGGATCC GATCTTCAAAGGTTGTGCTCAG |
AtaAupstF2 | AACGCAAGTTGTTTTACTGC |
AtaAupstR | CGCGGATCC TAGAAGCTGTAGCAGTTGTTCC |
AtaAdwstF | CGCGGATCC ACTCGACAGGGAAGATCTTC |
AtaAdwstR | CGCGGATCC AATTGAATCATCAACACCTGCTG |
AtaAdwstR2 | TACGTCGAGCAGCTAAGGTC |