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Figure 5 | BMC Microbiology

Figure 5

From: A new simple method for introducing an unmarked mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT recombination

Figure 5

Examination of the phenotype of the unmarked mutant Tol 5 4140. (A) Immunodetection of AtaA using an anti-AtaA antiserum against whole cell lysates prepared from Tol 5 WT and the 4140 mutant. (B) Growth curve of Tol 5 WT and the 4140 mutant in LB medium at 28°C, with shaking at 115 rpm. Data are expressed as the mean and SD obtained from 3 independent cultures. (C) Adhesion of Tol 5 WT and the 4140 mutant to a polystyrene surface. The photograph indicates the stained cells adhering to a 48-well plate. Data are expressed as the mean and SEM (n = 3). Statistical significance, *P < 0.01. (D) Autoagglutination assay of Tol 5 WT and the 4140 mutant by the tube-settling assay. The photographs indicate test tubes after a 3-h incubation without agitation. Data are expressed as the mean and SEM (n = 3). Statistical significance, *P < 0.001.

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BMC Microbiology

ISSN: 1471-2180

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