Figure 2
From: Rapid plasmid replicon typing by real time PCR melting curve analysis
Detection of multiple WT plasmids shows the same melting curves as corresponding cloned replicon controls. The left panel shows the melting curve of a WT strain with multiple plasmids. These plasmids were found to be of the ColE and F replicon. In the right panel the same result is obtained from two control strains each bearing either ColE or the F replicon. The melting temperature in the left panel peaks correspond exactly with the right panel peaks at 84.6°C and 86.4°C.