Detection of multiple WT plasmids shows the same melting curves as corresponding cloned replicon controls. The left panel shows the melting curve of a WT strain with multiple plasmids. These plasmids were found to be of the ColE and F replicon. In the right panel the same result is obtained from two control strains each bearing either ColE or the F replicon. The melting temperature in the left panel peaks correspond exactly with the right panel peaks at 84.6°C and 86.4°C.