Concentration of cells (CFU/ml)
|
Multiplex PNA-FISH assay
|
---|
L. crispatus
|
G. vaginalis 5-1
|
Lac663 Probe efficiency
|
Gard162 Probe efficiency
|
---|
1×109
|
1×109
|
+++
|
+++
|
1×105
|
1×105
|
+++
|
+++
|
1×103
|
1×103
|
++++
|
+++
|
L. iners
|
G. vaginalis 5-1
|
Lac663 Probe efficiency
|
Gard162 Probe efficiency
|
1×109
|
1×109
|
+++
|
+++
|
1×105
|
1×105
|
+++
|
+++
|
1×103
|
1×103
|
++
|
+++
|
- The PNA probe (Lac663 and Gar162) efficiencies were tested in each sample with the following hybridization PNA FISH qualitative evaluation: (++) Moderate hybridization; (+++) Good hybridization; (++++) Optimal hybridization. The table shows the median value from the three experiments for each sample.