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Table 4 Efficiency of the Lactobacillus spp. and G. vaginalis detection in adhesion assays with HeLa cell line

From: Fluorescence in situ Hybridization method using Peptide Nucleic Acid probes for rapid detection of Lactobacillus and Gardnerella spp.

Concentration of cells (CFU/ml) Multiplex PNA-FISH assay
L. crispatus G. vaginalis 5-1 Lac663 Probe efficiency Gard162 Probe efficiency
1×109 1×109 +++ +++
1×105 1×105 +++ +++
1×103 1×103 ++++ +++
L. iners G. vaginalis 5-1 Lac663 Probe efficiency Gard162 Probe efficiency
1×109 1×109 +++ +++
1×105 1×105 +++ +++
1×103 1×103 ++ +++
  1. The PNA probe (Lac663 and Gar162) efficiencies were tested in each sample with the following hybridization PNA FISH qualitative evaluation: (++) Moderate hybridization; (+++) Good hybridization; (++++) Optimal hybridization. The table shows the median value from the three experiments for each sample.