Table 2 Bacteria strains and plasmids used in this study
Strains or plasmids | Characteristics | Source or reference |
|---|---|---|
Escherichia coli | Â | Â |
DH5α | Transformation recipient strain | [47] |
S17-1λ | Conjugation donor strain | [48] |
C. crescentus | Â | Â |
NA1000 | Also CB15N, synchronizable derivative of wild-type CB15 | [49] |
MM46 | NA1000 ΔnczA (ΔCCNA_02473) | This work |
MM47 | NA1000 ΔczrA (ΔCCNA_02809) | This work |
MM48 | NA1000ΔczrAΔnczA | This work |
MM46+ | MM46 xylX::nczA | This work |
MM47+ | MM47 xylX::czrA | This work |
Plasmids | Â | Â |
pGEM-T Easy | Cloning vector; Ampr | Promega |
pRKlacZ290 | pRK2-derived vector with a promoterless lacZ gene; Tetr | [50] |
pNPTS138 | Suicide vector used for gene disruption containing oriT and sacB; Kanr | D. Alley |
pNPT228XNE | xylX locus in pNPT228; Kanr | [51] |