Figure 3From: Two RND proteins involved in heavy metal efflux in Caulobacter crescentus belong to separate clusters within proteobacteriaInduction of gene expression by divalent cations. The reporter lacZ gene expression driven by promoters Pczr and Pncz was evaluated by β-galactosidase activity assays in the presence of different divalent cations. The results shown are the average of at least three experiments. Error bars indicate standard deviations. Metal concentrations were: CdCl2, 10 μM; ZnCl2, 100 μM; CoCl2, 100 μM; NiCl2, 100 μM. Asterisks indicate results significantly different than those of of the same time points without metal (p ≤ 0.05).Back to article page