Table 3 Strains and plasmids used in this study

P. aeruginosa

 PA14 WT

Wild type P. aeruginosa PA14

[25]

 PA14 typA

typA insertion mutant of PA14, Gm^r

This study

 PA14 typA::ptypA⁺

Complemented mutant PA14 typA harboring plasmid pUCP20::typA⁺; Gm^r, Cb^r

This study

 PA14 pscC

pscC transposon mutant ID29579 of the Harvard PA14 mutant library

[25]

E. coli

 DH5α

F^–ϕ80lacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(r_K^– m_K⁺) supE44λ^–thi-1 gyrA96 relA

Invitrogen

Plasmids

 pUCP20

E. coli – Pseudomonas shuttle vector for constitutive expression of cloned genes, Cb^r

[42]

 pEX18Ap

Suicide vector for mutant regeneration in Pseudomonas, Amp^r/Cb^r

[43]

 pUCP20::typA⁺

pUCP20 containing the cloned typA gene; Amp^r/Cb^r

This study

 pUCP20::exsA⁺

pUCP20 containing the cloned exsA gene; Amp^r/Cb^r

This study