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Table 3 Strains and plasmids used in this study

From: TypA is involved in virulence, antimicrobial resistance and biofilm formation in Pseudomonas aeruginosa

Strain or plasmid Description and characteristicsa Reference
Strains   
P. aeruginosa   
 PA14 WT Wild type P. aeruginosa PA14 [25]
 PA14 typA typA insertion mutant of PA14, Gmr This study
 PA14 typA::ptypA+ Complemented mutant PA14 typA harboring plasmid pUCP20::typA+; Gmr, Cbr This study
 PA14 pscC pscC transposon mutant ID29579 of the Harvard PA14 mutant library [25]
E. coli   
 DH5α Fϕ80lacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK mK+) supE44λthi-1 gyrA96 relA Invitrogen
Plasmids   
 pUCP20 E. coli – Pseudomonas shuttle vector for constitutive expression of cloned genes, Cbr [42]
 pEX18Ap Suicide vector for mutant regeneration in Pseudomonas, Ampr/Cbr [43]
 pUCP20::typA+ pUCP20 containing the cloned typA gene; Ampr/Cbr This study
 pUCP20::exsA+ pUCP20 containing the cloned exsA gene; Ampr/Cbr This study
  1. a Antibiotic resistance phenotypes: Ampr, ampicillin resistance for E. coli; Cbr, carbenicillin resistance for P. aeruginosa; Gmr, gentamicin resistance.