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Table 3 The performance of the libraries considering the species and treatment applied to the clinical quadruplicates

From: Assessment of various parameters to improve MALDI-TOF MS reference spectra libraries constructed for the routine identification of filamentous fungi

  

Independent spots

Best of 4 spots

Clinical isolate MSP for 4 spots

Species

 

B0

B1

B2

B3

B4

B5

B6

B7

B0

B1

B2

B3

B4

B5

B6

B7

B0

B1

B2

B3

B4

B5

B6

B7

Alternaria alternata (10)

a

70

73

80

88

88

83

95

95

60

70

90

90

80

90

100

100

80

80

80

80

80

70

100

100

b

1.67

1.83

1.84

1.82

1.78

1.71

1.91

1.95

1.91

1.96

1.87

1.89

1.81

1.79

1.98

2.02

1.63

1.7

1.81

1.84

1.74

1.77

1.85

1.92

Aspergillus flavus (8)

a

91

78

81

88

88

94

94

100

88

88

100

100

100

100

100

100

88

75

63

63

75

88

88

100

b

1.58

1.64

1.68

1.73

1.65

1.72

1.74

2.01

1.8

1.76

1.73

1.77

1.73

1.8

1.83

2.09

1.54

1.66

1.93

1.95

1.77

1.77

2.02

2.03

Aspergillus fumigatus (85)

a

84

79

84

88

86

85

96

97

92

91

91

91

93

89

98

98

88

85

87

87

86

88

99

98

b

1.58

1.59

1.67

1.77

1.7

1.71

2.03

2.04

1.69

1.69

1.77

1.87

1.78

1.82

2.13

2.14

1.58

1.6

1.67

1.76

1.69

1.64

2.05

2.08

Aspergillus nidulans (2)

a

29

14

14

43

57

29

14

43

50

50

50

50

100

50

50

50

50

50

50

50

100

50

50

50

b

1.37

1.89

1.89

1.56

1.53

1.39

1.89

1.82

1.58

1.89

1.89

1.89

1.52

1.49

1.89

1.89

1.64

1.62

1.62

1.63

1.41

1.14

1.63

1.83

Aspergillus niger (12)

a

85

83

81

77

65

63

77

83

92

83

83

83

67

67

83

83

83

83

75

75

75

75

92

83

b

1.56

1.57

1.59

1.66

1.54

1.55

1.77

1.89

1.67

1.67

1.68

1.73

1.69

1.71

1.83

1.97

1.53

1.47

1.58

1.65

1.57

1.47

1.6

1.89

Aspergillus terreus (10)

a

28

25

33

35

28

25

55

63

30

30

40

40

40

40

60

70

50

40

50

50

50

40

70

70

b

1.23

1.14

1.19

1.3

1.22

1.22

1.67

1.61

1.35

1.29

1.36

1.41

1.41

1.35

1.79

1.7

1.06

1.17

1.14

1.2

1.21

1.24

1.66

1.66

Beauveria bassiana (1)

a

0

0

100

100

75

75

75

75

0

0

100

100

100

100

100

100

0

0

0

0

0

0

0

0

b

  

1.2

1.2

1.05

0.93

1.24

1.26

  

1.32

1.32

1.12

1.06

1.32

1.32

        

Fusarium oxysporum (2)

a

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

100

b

1.93

2.06

2.06

2.07

1.82

1.78

2.11

2.12

2

2.11

2.11

2.11

1.98

2

2.16

2.17

1.97

2.06

2.06

2.06

1.79

1.9

2.06

2.06

Microsporum audouinii (10)

a

45

33

30

30

40

33

30

65

60

50

50

50

40

40

50

70

50

50

50

50

30

40

50

80

b

1.49

1.4

1.44

1.57

1.35

1.47

1.59

1.8

1.59

1.54

1.55

1.7

1.64

1.67

1.7

1.91

1.41

1.2

1.38

1.45

1.59

1.33

1.54

1.71

Microsporum canis (1)

a

0

0

0

0

0

0

25

50

0

0

0

0

0

0

0

100

0

0

0

0

0

0

0

100

b

      

1.17

1.51

       

1.56

       

1.65

Penicillium aurantiogriseum/chrysogenum (8)

a

34

34

44

63

41

28

75

75

38

25

50

63

50

38

75

75

50

0

0

0

0

0

38

50

b

1.7

1.59

1.58

1.88

1.64

1.88

1.98

2

1.86

2.1

1.72

2.03

1.65

1.87

2.19

2.19

1.75

     

2.07

2.11

Paecilomyces variotii (1)

a

0

0

0

0

0

0

25

25

0

0

0

0

0

0

100

100

0

0

0

0

0

0

0

100

b

      

1.2

1.28

      

1.2

1.28

       

1.76

Rhizopus oryzae (3)

a

58

50

58

75

50

58

75

75

67

67

100

100

33

67

100

100

67

67

100

100

67

67

67

67

b

1.64

2.14

2.05

2.05

1.89

1.69

2.05

2.05

2.03

2.15

1.95

2.06

2.28

1.92

2.06

2.06

1.89

2.16

1.84

1.92

2.02

1.75

2.27

2.27

Scedosporium apiospermum (8)

a

47

44

41

47

44

41

56

66

50

50

50

63

38

38

50

63

50

50

63

63

38

63

63

75

b

1.53

1.33

1.45

1.56

1.59

1.52

1.62

1.67

1.69

1.63

1.65

1.71

1.96

1.81

1.84

1.9

1.97

1.83

1.88

1.9

2.26

1.81

1.96

2.02

Trichophyton mentagrophytes (2)

a

88

63

100

100

88

100

100

100

100

50

100

100

50

100

100

100

100

50

100

100

50

100

100

100

b

1.91

1.93

1.9

1.93

1.8

1.67

1.94

1.94

1.92

2.37

1.97

2.01

2.44

1.74

2.01

2.01

1.84

2.35

1.96

2.01

2.47

1.69

2.01

2.01

Trichophyton rubrum (8)

a

47

50

53

69

53

53

78

69

50

75

88

75

63

63

88

75

63

50

63

63

63

38

63

50

b

1.11

1.13

1.28

1.43

1.47

1.29

1.51

1.65

1.52

1.27

1.35

1.5

1.64

1.54

1.63

1.83

1.35

1.07

1.38

1.46

1.47

1.81

1.54

1.74

Trichophyton soudanense (6)

a

17

17

71

38

46

13

92

88

0

17

67

50

50

0

100

100

0

50

67

50

50

17

83

100

b

1.08

1.24

1.39

1.46

1.37

1.17

1.91

1.97

 

1.35

1.48

1.53

1.42

 

2

2.02

 

0.96

1.03

1.08

1.21

0.86

2

1.9

All species in the library (177)

a

68

64

70

74

70

67

83

87

73

72

80

80

75

72

88

90

73

69

72

72

69

68

84

88

b

1.56

1.58

1.64

1.73

1.65

1.65

1.92

1.96

1.7

1.7

1.73

1.82

1.75

1.78

2.01

2.05

1.58

1.57

1.64

1.72

1.67

1.63

1.94

2

Non-A. fumigatus species included in the library (92)

a

53

50

57

61

56

51

71

77

55

55

70

70

59

57

78

83

60

54

59

58

53

51

71

78

b

1.54

1.57

1.59

1.68

1.58

1.58

1.78

1.86

1.72

1.7

1.67

1.76

1.7

1.71

1.87

1.95

1.57

1.52

1.6

1.66

1.64

1.61

1.81

1.9

  1. a: percentage of concordant identification, b: LS mean of the concordant identifications.