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Table 3 The performance of the libraries considering the species and treatment applied to the clinical quadruplicates

From: Assessment of various parameters to improve MALDI-TOF MS reference spectra libraries constructed for the routine identification of filamentous fungi

   Independent spots Best of 4 spots Clinical isolate MSP for 4 spots
Species   B0 B1 B2 B3 B4 B5 B6 B7 B0 B1 B2 B3 B4 B5 B6 B7 B0 B1 B2 B3 B4 B5 B6 B7
Alternaria alternata (10) a 70 73 80 88 88 83 95 95 60 70 90 90 80 90 100 100 80 80 80 80 80 70 100 100
b 1.67 1.83 1.84 1.82 1.78 1.71 1.91 1.95 1.91 1.96 1.87 1.89 1.81 1.79 1.98 2.02 1.63 1.7 1.81 1.84 1.74 1.77 1.85 1.92
Aspergillus flavus (8) a 91 78 81 88 88 94 94 100 88 88 100 100 100 100 100 100 88 75 63 63 75 88 88 100
b 1.58 1.64 1.68 1.73 1.65 1.72 1.74 2.01 1.8 1.76 1.73 1.77 1.73 1.8 1.83 2.09 1.54 1.66 1.93 1.95 1.77 1.77 2.02 2.03
Aspergillus fumigatus (85) a 84 79 84 88 86 85 96 97 92 91 91 91 93 89 98 98 88 85 87 87 86 88 99 98
b 1.58 1.59 1.67 1.77 1.7 1.71 2.03 2.04 1.69 1.69 1.77 1.87 1.78 1.82 2.13 2.14 1.58 1.6 1.67 1.76 1.69 1.64 2.05 2.08
Aspergillus nidulans (2) a 29 14 14 43 57 29 14 43 50 50 50 50 100 50 50 50 50 50 50 50 100 50 50 50
b 1.37 1.89 1.89 1.56 1.53 1.39 1.89 1.82 1.58 1.89 1.89 1.89 1.52 1.49 1.89 1.89 1.64 1.62 1.62 1.63 1.41 1.14 1.63 1.83
Aspergillus niger (12) a 85 83 81 77 65 63 77 83 92 83 83 83 67 67 83 83 83 83 75 75 75 75 92 83
b 1.56 1.57 1.59 1.66 1.54 1.55 1.77 1.89 1.67 1.67 1.68 1.73 1.69 1.71 1.83 1.97 1.53 1.47 1.58 1.65 1.57 1.47 1.6 1.89
Aspergillus terreus (10) a 28 25 33 35 28 25 55 63 30 30 40 40 40 40 60 70 50 40 50 50 50 40 70 70
b 1.23 1.14 1.19 1.3 1.22 1.22 1.67 1.61 1.35 1.29 1.36 1.41 1.41 1.35 1.79 1.7 1.06 1.17 1.14 1.2 1.21 1.24 1.66 1.66
Beauveria bassiana (1) a 0 0 100 100 75 75 75 75 0 0 100 100 100 100 100 100 0 0 0 0 0 0 0 0
b    1.2 1.2 1.05 0.93 1.24 1.26    1.32 1.32 1.12 1.06 1.32 1.32         
Fusarium oxysporum (2) a 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100
b 1.93 2.06 2.06 2.07 1.82 1.78 2.11 2.12 2 2.11 2.11 2.11 1.98 2 2.16 2.17 1.97 2.06 2.06 2.06 1.79 1.9 2.06 2.06
Microsporum audouinii (10) a 45 33 30 30 40 33 30 65 60 50 50 50 40 40 50 70 50 50 50 50 30 40 50 80
b 1.49 1.4 1.44 1.57 1.35 1.47 1.59 1.8 1.59 1.54 1.55 1.7 1.64 1.67 1.7 1.91 1.41 1.2 1.38 1.45 1.59 1.33 1.54 1.71
Microsporum canis (1) a 0 0 0 0 0 0 25 50 0 0 0 0 0 0 0 100 0 0 0 0 0 0 0 100
b        1.17 1.51         1.56         1.65
Penicillium aurantiogriseum/chrysogenum (8) a 34 34 44 63 41 28 75 75 38 25 50 63 50 38 75 75 50 0 0 0 0 0 38 50
b 1.7 1.59 1.58 1.88 1.64 1.88 1.98 2 1.86 2.1 1.72 2.03 1.65 1.87 2.19 2.19 1.75       2.07 2.11
Paecilomyces variotii (1) a 0 0 0 0 0 0 25 25 0 0 0 0 0 0 100 100 0 0 0 0 0 0 0 100
b        1.2 1.28        1.2 1.28         1.76
Rhizopus oryzae (3) a 58 50 58 75 50 58 75 75 67 67 100 100 33 67 100 100 67 67 100 100 67 67 67 67
b 1.64 2.14 2.05 2.05 1.89 1.69 2.05 2.05 2.03 2.15 1.95 2.06 2.28 1.92 2.06 2.06 1.89 2.16 1.84 1.92 2.02 1.75 2.27 2.27
Scedosporium apiospermum (8) a 47 44 41 47 44 41 56 66 50 50 50 63 38 38 50 63 50 50 63 63 38 63 63 75
b 1.53 1.33 1.45 1.56 1.59 1.52 1.62 1.67 1.69 1.63 1.65 1.71 1.96 1.81 1.84 1.9 1.97 1.83 1.88 1.9 2.26 1.81 1.96 2.02
Trichophyton mentagrophytes (2) a 88 63 100 100 88 100 100 100 100 50 100 100 50 100 100 100 100 50 100 100 50 100 100 100
b 1.91 1.93 1.9 1.93 1.8 1.67 1.94 1.94 1.92 2.37 1.97 2.01 2.44 1.74 2.01 2.01 1.84 2.35 1.96 2.01 2.47 1.69 2.01 2.01
Trichophyton rubrum (8) a 47 50 53 69 53 53 78 69 50 75 88 75 63 63 88 75 63 50 63 63 63 38 63 50
b 1.11 1.13 1.28 1.43 1.47 1.29 1.51 1.65 1.52 1.27 1.35 1.5 1.64 1.54 1.63 1.83 1.35 1.07 1.38 1.46 1.47 1.81 1.54 1.74
Trichophyton soudanense (6) a 17 17 71 38 46 13 92 88 0 17 67 50 50 0 100 100 0 50 67 50 50 17 83 100
b 1.08 1.24 1.39 1.46 1.37 1.17 1.91 1.97   1.35 1.48 1.53 1.42   2 2.02   0.96 1.03 1.08 1.21 0.86 2 1.9
All species in the library (177) a 68 64 70 74 70 67 83 87 73 72 80 80 75 72 88 90 73 69 72 72 69 68 84 88
b 1.56 1.58 1.64 1.73 1.65 1.65 1.92 1.96 1.7 1.7 1.73 1.82 1.75 1.78 2.01 2.05 1.58 1.57 1.64 1.72 1.67 1.63 1.94 2
Non-A. fumigatus species included in the library (92) a 53 50 57 61 56 51 71 77 55 55 70 70 59 57 78 83 60 54 59 58 53 51 71 78
b 1.54 1.57 1.59 1.68 1.58 1.58 1.78 1.86 1.72 1.7 1.67 1.76 1.7 1.71 1.87 1.95 1.57 1.52 1.6 1.66 1.64 1.61 1.81 1.9
  1. a: percentage of concordant identification, b: LS mean of the concordant identifications.