Diversity of endophytic fungi and screening of fungal paclitaxel producer from Anglojap yew, Taxus x media

BMC Microbiology

Table 3 Oligonucleotide primers used in PCR screening

Gene (GenBank No.)	Primers	Sequence (5′-3′)	Amplicon length
ITS1-5.8S-ITS2 rDNA	ITS1	TCCGTAGGTGAACCTGCGG	500-600 bp [39]
	ITS4	TCCTCCGCTTATTGATATGC
ts (AY364469)	ts-F	CAAACCCATGTCGAATTGAGAAG	631 bp [16]
	ts-R	CAAGTTTGCATACACTCTGGAATCT
dbat (EF028093)	dbat-F	GGGAGGGTGCTCTGTTTG	153 bp [17]
	dbat-R	GTTACCTGAACCACCAGAGG
bapt (AY082804)	bapt-F	CCTCTCTCCGCCATTGACAA	453 bp [17]
	bapt-R	TCGCCATCTCTGCCATACTT

ISSN: 1471-2180