Table 1 Sequences of sense (S) and antisense (AS) primers used for real time-RT-PCR analysis (18)
From: Safety evaluation of the antimicrobial peptide bovicin HC5 orally administered to a murine model
Primers | Sequences |
|---|---|
β-actin S | 5′ AGC TGC GTT TTA CAC CCT TT 3′ |
β-actin AS | 5′ AAG CCA TGC CAA TGT TGT CT 3′ |
IL-10 S | 5′ TGG ACA ACA TAC TGC TAA CC 3′ |
IL-10 AS | 5′ GGA TCA TTT CCG ATA AGG CT 3′ |
IL-4 S | 5′ CTG ACG GCA CAG AGC TAT TGA 3′ |
IL-4 AS | 5′ TAT GCG AAG CAC CTT GGA AGC 3′ |
IL-5 S | 5′ GAG GTT ACA GAC ATG CAC CAT T 3′ |
IL-5 AS | 5′ TCA GTT GGT AAC ATG CAC AAA G 3′ |
IL-13 S | 5′ ACC AAC ATC TCC AAT TGC AA 3′ |
IL-13 AS | 5′ ATG CAA TAT CCT CTG GGT CC 3′ |
TNF-α S | 5′ TGT GCT CAG AGC TTT CAA CAA 3′ |
TNF-α AS | 5′ CTT GAT GGT GGT GCA TGA GA 3′ |
IL-12 p40 S | 5′ AGC ACC AGC TTC TTC ATC AGG 3′ |
IL-12 p40 AS | 5′ GCG CTG GAT TCG AAC AAA G 3′ |
IFN-γ S | 5′ GCA TCT TGG CTT TGC AGC T 3′ |
IFN-γ AS | 5′ CCT TTT TCG CCT TGC TGT TG 3′ |
TGF-β S | 5′ GCT GAA CCA AGG AGA CGG AAT 3′ |
TGF-β AS | 5′ GCT GAT CCC GTT GAT TTC CA 3′ |
IL-17 S | 5′ GCT CCA GAA GGC CCT CAG A 3′ |
IL-17 AS | 5′ CTT TCC CTC CGC ATT GAC A 3′ |