Table 2 Primers used for PCR
Primer | Sequence (5′-3′)a | Target/locationb | Reference |
|---|---|---|---|
acf-R1 | TCCTCAGCATCCTTTTCTTCA | acf | This study |
arsR-up1 | TGTGGATCTATGGAATTGAGGA | upstream of arsR | This study |
feoB-F1 | ATGGTCTCCAAAAAGCATGA | feoB | This study |
feoB-F2 | AAGACAGGAACAAGCGAAACA | feoB | This study |
feoB-R1 | TGGGGCATTGATTACACTGA | feoB | This study |
IRL-scc | TATCRGWTRATGATGMGGTTT | IRL of SCCmec | [7] |
IS2 | TGAGGTTATTCAGATATTTCGATGT | IS431 | [31] |
MecA147-F | GTGAAGATATACCAAGTGATT | mecA | [30] |
MecA147-R | ATGCGCTATAGATTGAAAGGA | mecA | [30] |
orf_1-F1 | GAAATGAGCTGAAAGCACGA | lip | This study |
Orf2_1-R1 | TTGGAGGTTTCTCCCCATC | orf24, a Putative multicopper oxidases gene | This study |
Orf24-1 | CCAAATGAATTGTTAGACGTTG | spacer between putP and IS431Δ | This study |
orfX-F1 | GAAAAAGCACCWGAAAMTATGAG | orfX | [7] |
SH0128-R1 | TTTTGTGGTTGTGACGGTGT | orf45, locus SH0128 in AP006716 | This study |
ZZ-3 | GTGAGGTTGGTGGTGATAAAA | spacer between putP and IS431Δ | This study |
ZZ-4 | GCGGGTCCTTCTGGTATAGG | FAD | This study |
ZZ-11 | TATCTCGGGAAATCGATAAAAA | spacer between IS431 and orf32 | This study |
ZZ-12 | GTTGAAAGGAAACAAAAACTACG | spacer between IS431 and orf32 | This study |
ZZ-16 | CCGATAACGTCATTCCATCT | orf32, ABC-type bacteriocin transporter gene | This study |
ZZ-24 | AGCACGACAACAAAAGCATC | orf33 | This study |
ZZ-28 | TGGAGGAGGAGTTTTGGCTA | orf35, chromate transporter | This study |
ZZ-29 | TACGACATGACCACCTCCAA | orf35, chromate transporter | This study |
ZZ-30 | GTAGCTGTTGCCATTGTTGC | orf35, chromate transporter | This study |
ZZ-31 | GCTTGCAGGTCCAGGTAAAA | orf35, chromate transporter | This study |
ZZ-32 | TGGACGTATCGCTTCAAATG | orf33 | This study |