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Table 1 Genes and MGE in the genetic context of mecA in WCH1

From: Characterization of a complex context containing mecA but lacking genes encoding cassette chromosome recombinases in Staphylococcus haemolyticus

Gene or MGE

Position a

Product

Closest match b,c

Identity, species strain

orfX

1-316

Hypothetical protein

99%, S. haemolyticus JCSC1435

ADP

445-1431

ADP-ribosylglycohydrolase

99%, S. epidermidis RP62a (locus SERP2218)

perM

1450-2784

Cytosine/purines, uracil, thiamine, allantoin permease family protein

99%, S. epidermidis RP62a (locus SERP2217)

rbkΔd

2781-3719

Ribokinase

99%, S. epidermidis RP62a (locus SERP2216)

IS431

3701-4401

IS431

 

merR

4888-5235

Transcriptional regulator of the merR family

100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0094)

thiJ

5313-5996

ThiJ/PfpI family protein

100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0095)

orf8

6018-6683

NAD dependent epimerase/dehydratase family protein

100%, type IX SCCmec of S. aureus JCSC6943, type X SCCmec of S. aureus JCSC6945 and S. haemolyticus JCSC1435 (locus SH0095)

orf9

6687-7691

Oxidoreductase, zinc-binding dehydrogenase family protein

100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0097)

orf10

7700-8065

Hypothetical protein of the COG4270 superfamily, predicted membrane protein

100%, type IX SCCmec of S. aureus JCSC6943, type X SCCmec of S. aureus JCSC6945 and S. haemolyticus JCSC1435 (locus SH0098)

cadD

8601-9218

Cadmium binding protein

100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0099)

cadX

9237-9578

Cadmium resistant accessory protein

100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0100)

arsC

9999-9598

Arsenate reductase

100%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0101)

arsB

11306-10017

Arsenical pump membrane protein

99%, type IX SCCmec of S. aureus JCSC6943 and S. haemolyticus JCSC1435 (locus SH0102)

arsR

11623-11302

Arsenical resistance operon repressor

100%, type IX SCCmec of S. aureus JCSC6943, type X SCCmec of S. aureus JCSC6945 and S. haemolyticus JCSC1435 (locus SH0103)

IS431

11697-12486

IS431

 

mecRΔ

12503-12487

Signal transducer protein

 

mecA

12603-14609

Penicillin binding protein 2a

 

orf19

15083-14655

Hypothetical protein

 

maoC

15923-15180

Putative acyl dehydratase maoc

 

orf21

17208-16840

Putative HMG-CoA synthase (partial)

 

IS431

17209-17998

IS431

 

copA

18241-20262

Copper-transporting atpase

99%, type X SCCmec of S. aureus JCSC6945.

orf24

20277-21710

Putative multicopper oxidases

99%, S. haemolyticus JCSC1435 (locus SH0106)

lip

21730-22212

Lipoprotein

99%, S. aureus JCSC6943

acf

22588-23073

Putative Acyl-CoA acyltransferase

97%, S. haemolyticus JCSC1435 (locus SH0117)

hsdR

23254-23667

Type I restriction endonuclease, HsdR

97%, S. haemolyticus JCSC1435(locus SH0118)

putP

25274-23736

Sodium/proline symporter (High affinity proline permease)

78%, S. saprophyticus ATCC 15305 (locus SSP0399)

IS431Δ

26462-27184

IS431, truncated

 

FAD

27261-28382

FAD-dependent pyridine nucleotide-disulphide oxidoreductase

66%, a few S. aureus strains, e.g. COL

feoB

28376-29272

FeoB family ferrous iron transporter

68% (partially, from position 28804 to 29216), S. carnosus TM300

orf31

29337-29717

Putative transmembrane protein

73% (partially, from position 29438 to 29618), S. aureus MSHR1132

IS431Δe

30690-29891

IS431, incomplete due to internal termination

 

orf32

31660-33822

ABC-type bacteriocin transporter family protein

71%, S. epidermidis plasmid SAP105A

orf33

34541-35809

DUF867 type protein, putative phage-related protein

71% (partially from position 35252), S. epidermidis ATCC 12228

ISSha1

37543-36061

ISSha1

98%, S. haemolyticus JCSC1435

chr

38832-37669

Chromate transporter

66% (partially from position 37895 to 38782), Oceanobacillus iheyensis HTE831

arsC

39261-38869

Arsenate reductase

97%, S. aureus strains LGA251 and M10/0061

arsB

40577-39279

Arsenical pump membrane protein

92%, S. xylosus plasmid pSX267

arsR

40885-40571

Arsenical resistance operon repressor

91%, S. aureus plasmid SAP099B and EDINA

orf39

41223-41771

DUF576 type protein

100%, S. haemolyticus JCSC1435 (locus SH0120)

orf40

41768-41935

Hypothetical protein

100%, S. haemolyticus JCSC1435 (locus SH0121)

orf41

42126-43013

Hypothetical protein, similar to mechanosensitive ion channel Mscs, transmembrane protein

100%, S. haemolyticus JCSC1435 (locus SH0122)

orf42

43522-44046

DUF3267 type protein

100%, S. haemolyticus JCSC1435 (locus SH0123)

orf43

44998-44120

Hypothetical protein, similar to cobalamin synthesis related protein CobW

100%, S. haemolyticus JCSC1435 (locus SH0124)

orf44

45625-46248

Hypothetical protein, similar to Zn-binding lipoprotein AdcA

100%, S. haemolyticus JCSC1435 (locus SH0125)

  1. a Positions are according to GenBank accession no. JQ764731.
  2. b GenBank accession no.: S. aureus LGA251 (FR821779), S. aureus JCSC6943 (AB505628), S. aureus JCSC6945 (AB505630), S. aureus M10/0061 (FR823292), S. aureus MSHR1132 (FR821777), S. carnosus TM300 (AM295250), S. epidermidis ATCC 12228 (AE015929), S. epidermidis RP62a (CP000029), S. haemolyticus JCSC1435 (AP006716), S. saprophyticus ATCC 15305 (AP008934), Oceanobacillus iheyensis HTE831 (BA000028), S. aureus plasmid SAP099B (GQ900449), S. aureus plasmid EDINA (AP003089), S. epidermidis plasmid SAP105A (GQ900452), S. xylosus plasmid pSX267 (M80565).
  3. c Closest matches of MGE (IS431 and ISSha1) and genes belonging to the mec complex are not listed as there are many identical matches.
  4. d Truncated by IS431 with 19 bp of the 3′ end missing and the read frame extending into IS431.
  5. e The tnpA of IS431 was terminated prematurely due to internal point mutation.