Skip to main content
Figure 2 | BMC Microbiology

Figure 2

From: Trans-species activity of a nonself recognition domain

Figure 2

Insertion of constructs into the GAL1 locus allows for control of trans-gene expression level. A) We examined proper regulation of our constructs by assessing the minimum inhibitory concentration (MIC) of hygromycin B. When grown in medium containing glucose (YPD), the Y2454 wild-type and control yeast strains had similar MIC values that were significantly greater than that of the PA-expressing strain (P = 0.017). B) In contrast, when grown in medium containing galactose (YPRaf/Gal), a significantly higher MIC was observed for yeast strains containing hph constructs, as compared to the wild-type strain (P < 0.001) and there was no significant difference in MIC values of control and PA-expressing strains. Error bars in panels A and B indicate standard deviation based on 5 biological replicates.

Back to article page