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Figure 1 | BMC Microbiology

Figure 1

From: Trans-species activity of a nonself recognition domain

Figure 1

Incompatibility activity is determined by the C-terminus of UN-24. A) Regions of un-24PA and un-24OR were fused to the hygromycin resistance gene (hph) and tested for incompatibility activity by transformations of PA [C2(2)-1] and OR (C9-2) strains. The red (PA) or purple (OR) region at the right represents the highly variable C-terminus region. At the right of each construct, “+*” indicates PA-like activity, “–” represents no incompatibility activity, “+” designates strong OR-like activity, and “+/−” indicates weak OR-like activity. Each interval on the bottom scale bar represents a length of 100 amino acid residues. B) Representative transformation assays of incompatible and compatible interactions in N. crassa. Transformation of un-24PA constructs into the OR (C9-2) strain resulted in ‘star’ colonies that are characteristic of PA-like incompatibility. In contrast, transformation of un-24OR into the PA [C2(2)-1] strain results in near complete cell death of the recipient strain and recovery of few or no transformants, indicative of strong OR-like incompatibility.

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