A high throughput multiplex PCR assay for simultaneous detection of seven aminoglycoside-resistance genes in Enterobacteriaceae

Table 5 Comparison of GeXP assay and conventional single PCR for detecting seven aminoglycoside-resistance genes

Resistance genes	GeXP assay	Conventional single PCR			Measures of agreement
Resistance genes	GeXP assay	Positive	Negative	Total	Kappa values*
aac(3)-II	Positive	37	2	39	0.831 (P=0.000)
	Negative	2	15	17
	Total	39	17	56
aac(6')-Ib	Positive	11	3	14	0.846 (P=0.000)
	Negative	0	42	42
	Total	11	45	56
aac(6')-II	Positive	8	2	10	0.810 (P=0.000)
	Negative	1	45	46
	Total	9	47	56
ant(3″)-I	Positive	40	2	42	0.909 (P=0.000)
	Negative	0	14	14
	Total	40	16	56
aph(3')-VI	Positive	10	0	10	0.887 (P=0.000)
	Negative	2	44	46
	Total	12	44	56
armA	Positive	40	2	42	0.810 (P=0.000)
	Negative	2	12	14
	Total	42	14	56
rmtB	Positive	14	3	17	0.825 (P=0.000)
	Negative	1	38	39
	Total	15	41	56

*As a rule of thumb, values of Kappa from 0.40 to 0.59 are considered moderate, 0.60 to 0.79 substantial, and 0.80 outstanding.

ISSN: 1471-2180