Figure 3

The sensitivity of GeXP assay for detection of seven aminoglycoside-resistance genes. The GeXP assay was carried out using different concentrations of seven premixed recombinant plasmids with 1000 copies (a), 100 copies (b), 10 copies (c) and 5 copies (d), respectively. All of seven aminoglycoside-resistance genes could be detected at 1000, 100 and 10 copies levels (a, b and c); only aac(6′)-II (217 bp) and ant(3″)-I (321 bp) could be detected at 5 copies levels in the optimized GeXP assay (d).