Skip to main content
Figure 4 | BMC Microbiology

Figure 4

From: Mechanistic studies of intracellular delivery of proteins by cell-penetrating peptides in cyanobacteria

Figure 4

Cell viability of the R9/GFP delivery system in the presence of uptake modulators. (A) The MTT assay. The 6803 strain of cyanobacteria was treated with BG-11 medium as a negative control, or treated with 100% methanol as a positive control. In the presence of NEM, cells were treated with R9/GFP complexes in the presence of CytD, EIPA, or wortmannin (Wort), respectively, and analyzed by the MTT assay. Significant differences were determined at P < 0.01 (**). Data are presented as mean ± SD from nine independent experiments. (B) The membrane leakage assay by a two-color fluorescence assay. The 6803 strain of cyanobacteria was treated with the same conditions in (A). SYTO 9 stains nucleic acids of live and dead cells in the GFP channel, while SYTOX blue stains nucleic acids of membrane-damaged cells in the BFP channel. Blue and green fluorescence were detected in BFP and GFP channels using a Leica confocal microscope at a magnification of 630×.

Back to article page