Analysis of toll-like receptors (TLRs) expression in bovine intestinal epithelial (BIE) cells. (A) TLR1-10 mRNA levels in BIE cells. The expression of TLR in BIE cells was calculated first as relative units compared to bovine β-actin level. After calculating the relative unit to β-actin, TLR1 was set as 1. Values represent means and error bars indicate the standard deviations. The results are means of six independent experiments. (B) Immunofluorescent localization of TLR2 and TLR4 in BIE cells. Green images indicate bovine TLR2 or TLR4 positive cells and nuclei in all panels were stained with DAPI (blue). Control experiments were performed by omitting the primary antibody. The results represent six independent experiments.