Figure 2

Relationships of the non-O1/non-O139 Vibrio cholerae isolates. A. Dendrogram analysis generated using the unweighted pair group method with arithmetic based on pulsed field gel electrophoresis (PFGE) patterns. Place corresponds to different cities in Zhejiang province: HZ - Hangzhou; JH - Jinhua; LS - Lishui; NB - Ningbo; QZ - Quzhou; SX - Shaoxing; TZ - Taizhou; and WZ – Wenzhou. The classification of the PFGE type (PT), sequence type (ST); presence (+) or absence (−) of the two T3SS genes (vcsC2 and vcsV2); and resistance (R) or intermediate (I) to antibiotics (E - erythromycin, TET - tetracycline, SXT - sulphamethoxazole/trimethoprim, CIP – ciprofloxacin, AMP – ampicillin, NA - nalidixic acid and RD – rifampicin) is shown. B. Minimum spanning tree based on MLST data. The number in the circle indicates the ST and the size of the circle corresponds the total number of isolates belonging to that ST. Different localities are indicated in colour and specified in the colour legend together with the total number of isolates from each city in brackets. City name abbreviations are the same as in A above. The number of allelic difference between STs is indicated on the branches. Nodes were connected by a dashed line if the difference is more than two alleles.