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Table 1 Microarray and qRT-PCR analysis of the genes that were differentially affected in the gatifloxacin resistant mutants, NCTR R and 13124 R

From: Comparative transcription analysis and toxin production of two fluoroquinolone-resistant mutants of Clostridium perfringens

Gene ID and name Function Microarray qRT-PCR
   mt/wt mt/wt
   NCTR ATCC 13124 NCTR ATCC 13124
Cell envelope
CPE1089 CPF_1345 putative membrane protein 4.3 −2.1 7.3 −2.8
CPE0162 CPF_0155 (pfoR) putative membrane protein 2.6 −4.0 3.3 −3.5
CPE0251 CPF_0244 putative lipoprotein 5.0 −2.4 2.0 −3.5
CPE0278 CPF_0274 (sagA) sagA protein 1.1 −2.4 4.7 −2.6
CPE0714 CPF_0710 putative monogalactosyl-diacylglycerol synthase 2.4 −2.4 7.6 6.3
Cellular processes
CPE0036 CPF_0042 (plc) phospholipase C 4.8 −6.8 1.9 −3.3
CPE0846 CPF_0840 (cloS1) α-clostripain 17.3 −15.6 8.3 −1143
CPE1474 CPF_1725 (hlyC) hemolysin III 3.2 −1.8 15.1 −2.6
CPE0163 CPF_0156 (pfoA) perfringolysin O 3.6 −71.4 6.4 −462
CPE0782 CPF_0784 (ahpC) alkyl hydroperoxide reductase-C subunit 10.3 −2.6 13.4 −12.6
CPE1092 CPF_1348 (pac) choloylglycine hydrolase family protein 1.7 −2.5 25.7 −1.7
Energy metabolism
CPE0778 CPF_0780 oxidoreductase, FDA-binding 4.8 −2.8 85 2.6
CPE1299 CPF_1505 (eno) enolase 3.5 −1.6 11.9 −1.9
CPE2058 CPF_2315 (gadB) glutamate decarboxylase 31.9 −3.5 20.0 −3.4
CPE2437 CPF_2747 (nrdH) glutaredoxin-like protein, YruB-family 3.8 −2.5 4.8 −11.0
CPE2551 CPF_2875 (glpA) probable glycerol-3-phosphate dehydrogenase 0.8 −2.5 1.3 −0.1
Purines, pyrimidines, nucleotides, and nucleosides
CPE2276 CPF_2558 (guaB) inosine-5’-monophosphate dehydrogenase 9.2 −3.6 30.3 −1.5
CPE2622 CPF_2958 (purA) adenylosuccinate synthetase 4.3 −1.9 14.8 −0.8
Protein fate
CPE0173 CPF_0166 (colA) collagenase 9.9 −4.7 8.5 −2.7
CPE2323 CPF_2632 (pepF) probable oligoendopeptidase F 2.7 -2.0 11.6 4.3
CPE1205 CPF_1002 (abgB) amidohydrolase family protein 1.9 −4.3 67.4 −1.6
Regulatory functions
CPE0073 CPF_0069 transcription antiterminator 2.1 −5.0 1.9 −2.6
CPE0759 CPF_0753 putative regulatory protein 1.5 −5.4 3.3 0.6
CPE1533 CPF_1784 (scrR) sucrose operon repressor 1.7 −2.8 132 −1.5
CPE2035 CPF_2292 (hrcA) heat-inducible transcription repressor HrcA 2.3 −2.9 9.5 5.5
CPE2363 CPF_2673 two-component sensor histidine kinase 2.1 −3.0 16.1 2.7
Transport and binding proteins
CPE1240 CPF_1450 (mgtE) magnesium transporter 8.6 −1.7 5.2 −2.6
CPE1300 CPF_1507 (gadC) glutamate:γ-aminobutyrate antiporter family protein 9.6 −2.7 17.1 −7.3
CPE1505 CPF_1756 (uraA) uracil transporter 3.8 −2.7 3.9 −4.6
CPE0075 CPF_0070 N-acetyl glucosamine-specific 1.4 −14.3 1 .8 ND
CPE0707 CPF_0703 ABC transporter, ATP-binding protein 1.5 −3.2 5.2 2.9
CPE0761 CPF_0756 (gltP) proton/sodium-glutamate symporter 1.5 −4.2 4.6 0.9
CPE1371 CPF_1621 sodium:neurotransmitter symporter family protein 1.8 −4.0 15.2 2.7
CPE2084 CPF_2341 (modB) molybdate ABC transporter, permease protein 1.8 −2.5 10.8 2.0
CPE2343 CPF_2652 (malE) putative maltose/maltodextrin ABC transporter 2.9 1.3 3.8 −2.1
Unknown functions
CPE0183 CPF_0176 nitroreductase family protein 1.0 −4.8 2.9 −1.1
CPE1172 CPF_1375 haloacid dehalogenase 2.1 −2.4 20.6 −1.7
CPE1784 CPF_2038 (nifU) NifU family protein 1.3 −2.5 6.4 −1.5
CPE2448 CPF_2758 PSP1 domain-containing protein 1.0 −2.4 5.5 −1.9
  1. All of the data are the means of three different experiments.
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