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Table 1 Microarray and qRT-PCR analysis of the genes that were differentially affected in the gatifloxacin resistant mutants, NCTR R and 13124 R

From: Comparative transcription analysis and toxin production of two fluoroquinolone-resistant mutants of Clostridium perfringens

Gene ID and name

Function

Microarray

qRT-PCR

  

mt/wt

mt/wt

  

NCTR

ATCC 13124

NCTR

ATCC 13124

Cell envelope

CPE1089 CPF_1345

putative membrane protein

4.3

−2.1

7.3

−2.8

CPE0162 CPF_0155 (pfoR)

putative membrane protein

2.6

−4.0

3.3

−3.5

CPE0251 CPF_0244

putative lipoprotein

5.0

−2.4

2.0

−3.5

CPE0278 CPF_0274 (sagA)

sagA protein

1.1

−2.4

4.7

−2.6

CPE0714 CPF_0710

putative monogalactosyl-diacylglycerol synthase

2.4

−2.4

7.6

6.3

Cellular processes

CPE0036 CPF_0042 (plc)

phospholipase C

4.8

−6.8

1.9

−3.3

CPE0846 CPF_0840 (cloS1)

α-clostripain

17.3

−15.6

8.3

−1143

CPE1474 CPF_1725 (hlyC)

hemolysin III

3.2

−1.8

15.1

−2.6

CPE0163 CPF_0156 (pfoA)

perfringolysin O

3.6

−71.4

6.4

−462

CPE0782 CPF_0784 (ahpC)

alkyl hydroperoxide reductase-C subunit

10.3

−2.6

13.4

−12.6

CPE1092 CPF_1348 (pac)

choloylglycine hydrolase family protein

1.7

−2.5

25.7

−1.7

Energy metabolism

CPE0778 CPF_0780

oxidoreductase, FDA-binding

4.8

−2.8

85

2.6

CPE1299 CPF_1505 (eno)

enolase

3.5

−1.6

11.9

−1.9

CPE2058 CPF_2315 (gadB)

glutamate decarboxylase

31.9

−3.5

20.0

−3.4

CPE2437 CPF_2747 (nrdH)

glutaredoxin-like protein, YruB-family

3.8

−2.5

4.8

−11.0

CPE2551 CPF_2875 (glpA)

probable glycerol-3-phosphate dehydrogenase

0.8

−2.5

1.3

−0.1

Purines, pyrimidines, nucleotides, and nucleosides

CPE2276 CPF_2558 (guaB)

inosine-5’-monophosphate dehydrogenase

9.2

−3.6

30.3

−1.5

CPE2622 CPF_2958 (purA)

adenylosuccinate synthetase

4.3

−1.9

14.8

−0.8

Protein fate

CPE0173 CPF_0166 (colA)

collagenase

9.9

−4.7

8.5

−2.7

CPE2323 CPF_2632 (pepF)

probable oligoendopeptidase F

2.7

-2.0

11.6

4.3

CPE1205 CPF_1002 (abgB)

amidohydrolase family protein

1.9

−4.3

67.4

−1.6

Regulatory functions

CPE0073 CPF_0069

transcription antiterminator

2.1

−5.0

1.9

−2.6

CPE0759 CPF_0753

putative regulatory protein

1.5

−5.4

3.3

0.6

CPE1533 CPF_1784 (scrR)

sucrose operon repressor

1.7

−2.8

132

−1.5

CPE2035 CPF_2292 (hrcA)

heat-inducible transcription repressor HrcA

2.3

−2.9

9.5

5.5

CPE2363 CPF_2673

two-component sensor histidine kinase

2.1

−3.0

16.1

2.7

Transport and binding proteins

CPE1240 CPF_1450 (mgtE)

magnesium transporter

8.6

−1.7

5.2

−2.6

CPE1300 CPF_1507 (gadC)

glutamate:γ-aminobutyrate antiporter family protein

9.6

−2.7

17.1

−7.3

CPE1505 CPF_1756 (uraA)

uracil transporter

3.8

−2.7

3.9

−4.6

CPE0075 CPF_0070

N-acetyl glucosamine-specific

1.4

−14.3

1 .8

ND

CPE0707 CPF_0703

ABC transporter, ATP-binding protein

1.5

−3.2

5.2

2.9

CPE0761 CPF_0756 (gltP)

proton/sodium-glutamate symporter

1.5

−4.2

4.6

0.9

CPE1371 CPF_1621

sodium:neurotransmitter symporter family protein

1.8

−4.0

15.2

2.7

CPE2084 CPF_2341 (modB)

molybdate ABC transporter, permease protein

1.8

−2.5

10.8

2.0

CPE2343 CPF_2652 (malE)

putative maltose/maltodextrin ABC transporter

2.9

1.3

3.8

−2.1

Unknown functions

CPE0183 CPF_0176

nitroreductase family protein

1.0

−4.8

2.9

−1.1

CPE1172 CPF_1375

haloacid dehalogenase

2.1

−2.4

20.6

−1.7

CPE1784 CPF_2038 (nifU)

NifU family protein

1.3

−2.5

6.4

−1.5

CPE2448 CPF_2758

PSP1 domain-containing protein

1.0

−2.4

5.5

−1.9

  1. All of the data are the means of three different experiments.