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Figure 4 | BMC Microbiology

Figure 4

From: The role of interactions between bacterial chaperone, aspartate aminotransferase, and viral protein during virus infection in high temperature environment: the interactions between bacterium and virus proteins

Figure 4

Thermodynamic characterization of the VP371-GroEL-aspartate aminotransferase (AST) interactions. The purified proteins of VP371-GroEL-AST linear complex and GST as control group were combined for isothermal titration calorimetry measurements. The experiment was performed at 25°C in phosphate buffered saline (pH 7.4) with 10-μL injections. (A) Thermogram (left) and binding isotherm (right) for the interaction between AST and GroEL. Concentrations of AST and GroEL were 44.5 and 8.5 μM, respectively. (B) Thermogram (left) and binding isotherm (right) for the interaction between VP371 and GroEL. Concentrations of VP371 and GroEL were 38.5 and 6.5 μM, respectively. (C) Thermogram for the titrations of 38.5 μM VP371 to 7 μM AST, 44.5 μM AST to 8.5 μM GST, 38.5 μM VP371 to 6.5 μM GST, and 44.5 μM GST to8.5 μM GroEL. (D) Thermodynamic parameters for binding of aspartate aminotransferase-GroEL and VP371-GroEL at different temperatures. All experiments were performed in phosphate buffered saline (pH 7.4) using isothermal titration calorimetry. Site 1–3, three different sites of the simulated interaction.

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