Population snapshot of S. agalactiae constructed in eBURST. In addition to the 9 eBURST groups that are shown, 36 singletons were present in the database (last accessed 7 November 2012). Founders of major clonal complexes (ST1, ST17, ST19, all of which form part of eBURST group 1, and ST23, which is the founder of eBURST group 2) and sequence types (ST) identified in the current study are labelled. Italics indicate STs isolated from fish, bold italics indicate the ST from fish and a frog, and shaded labels indicate STs from sea mammals. All β-haemolytic S. agalactiae isolates from fish belonged to a single branch of eBURST group 1, all seal isolates (n=6) belonged to eBURST group 2 and all non-haemolytic isolates belonged to two small eBURST groups that included ST260 and ST261.