Figure 7

Bacterial colonization by wild type and bioengineered L. jensenii sustained for 72 h does not alter levels of inflammation-associated proteins. Levels of immune mediators measured in cell culture supernatants by MSD multiplex after colonization of vaginal epithelial cells to by L. jensenii 1153 and modified Cyanovirin-N (mCV-N) expressing 1153–1666 for 72 h or after 24 h exposure to MALP-2 and subsequent change of medium at 24 h, 48 h, and 72 h. Bars represent mean and SEM from duplicate cultures in four independent experiments. ***P<0.001, ** P<0.01, * P<0.05 different from medium control, ⁺⁺⁺P<0.001,⁺⁺P<0.01, ⁺P<0.05 different from L. jensenii WT.