Figure 4

Cytokine profiles induced by bacteria or synthetic TLR2/6 ligand in cervicovaginal colonized epithelial model. Similar IL-8 levels measured in supernatants derived from primary and immortalized epithelial cells cultured with L. jensenii 1153–1666, 3666, gfp bioengineered and L. jensenii 1153 wild type (WT) strains or MALP-2 50 nM as a positive control. (Figure 4a) Two independent experiments with (VEC-100™) primary ectocervical originated tissue. (Figure 4b) Vaginal (Vk2/E6E7) and endocervical (End1/E6E7) epithelial colonized cells in one representative of three experiments. Bars represent mean and SEM from duplicate cultures. *** P<0.001 different from medium control, ⁺⁺⁺P<0.001 different from L. jensenii WT.