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Table 2 Material used in this study

From: Identification of genes involved in serum tolerance in the clinical strain Cronobacter sakazakiiES5

Strains/plasmids/primers Genotype/characteristic(s)/sequences Source or reference
Strains   
Cronobacter sakazakii   
  ES5 (wild type) Human isolate Hartmann et al., 2010, Johler et al., 2010 [11, 13]
  BF4 (mutant) ΔESA_04103, KanR Hartmann et al., 2010 [13]
  BF4_pCCR9 BF4 harboring pCCR9, KanR, TetR This study
  BF4_pCCR9::ESA_04103 BF4 harboring pCCR9:: ESA_04103, KanR, TetR This study
  21_G1 (mutant) ΔybaJ, KanR This study
Escherichia coli DH5 alpha F– Φ 80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1 Epicentre
Plasmids   
  pUC19 High copy cloning/expression vector AmpR Epicentre
  pCCR9 Low copy cloning/expression vector, TetR Randegger et al., 2000 [28]
  pCCR9::ESA_04103 pCCR9::ESA_04103, TetR This study
Primer for sequencing   
KAN-2FP1 5-ACC TAC AAC AAA GCT CTCATC AAC C-3 Epicentre
pCCR9-F 5-TTT GAC AGC TTA TCA TCG-3 This study
pCCR9-R 5-CCT ATG GAA GTT GAT CAG-3 This study
Primer for complementation   
  BF4f1 5-GAC GCC AAG CTT GCG CGA GCC TGC GTT TAA-3 This study
  BF4r2 5- AGT CTG GGA TCC AAA CAT TAT CCT TCT TTA TAG-3 This study
Target/primer for expression   
  ybaJf 5-CGG CAT GAT ATA GCG CAG-3 This study
  ybaJr 5-GAT GTG TAT AAG AGA CAG-3 This study
  hhaf 5-CAA ACC ATT AAC CAA AAC CG-3 This study
  hhar 5-CGG AAT TTT ATC GTA GAG CTT-3 This study
  fimAf 5-AAA CCG CGT TTA CTG G-3 This study
  fimAr 5-GCA ACG GAG TTT GCT T-3 This study
  16S rRNAf 5-GTG TTG TGA AAT GTT GGG T-3 This study
  16S rRNAr 5-ACT AGC GAT TCC GAC TT-3 This study
  1. 1 Primer sequence is underlined, recognition site for restriction enzyme Hind III is given in bold.
  2. 2 Primer sequence is underlined, recognition site for restriction enzyme Bam HI is given in bold.