Table 2 Material used in this study
From: Identification of genes involved in serum tolerance in the clinical strain Cronobacter sakazakiiES5
Strains/plasmids/primers | Genotype/characteristic(s)/sequences | Source or reference |
|---|---|---|
Strains | Â | Â |
Cronobacter sakazakii | Â | Â |
  ES5 (wild type) | Human isolate | |
  BF4 (mutant) | ΔESA_04103, KanR | Hartmann et al., 2010 [13] |
  BF4_pCCR9 | BF4 harboring pCCR9, KanR, TetR | This study |
  BF4_pCCR9::ESA_04103 | BF4 harboring pCCR9:: ESA_04103, KanR, TetR | This study |
  21_G1 (mutant) | ΔybaJ, KanR | This study |
Escherichia coli DH5 alpha | F– Φ 80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1 | Epicentre |
Plasmids | Â | Â |
  pUC19 | High copy cloning/expression vector AmpR | Epicentre |
  pCCR9 | Low copy cloning/expression vector, TetR | Randegger et al., 2000 [28] |
  pCCR9::ESA_04103 | pCCR9::ESA_04103, TetR | This study |
Primer for sequencing | Â | Â |
KAN-2FP1 | 5′-ACC TAC AAC AAA GCT CTCATC AAC C-3′ | Epicentre |
pCCR9-F | 5′-TTT GAC AGC TTA TCA TCG-3 | This study |
pCCR9-R | 5′-CCT ATG GAA GTT GAT CAG-3 | This study |
Primer for complementation | Â | Â |
  BF4f1 | 5′-GAC GCC AAG CTT GCG CGA GCC TGC GTT TAA-3′ | This study |
  BF4r2 | 5′- AGT CTG GGA TCC AAA CAT TAT CCT TCT TTA TAG-3′ | This study |
Target/primer for expression | Â | Â |
  ybaJf | 5′-CGG CAT GAT ATA GCG CAG-3′ | This study |
  ybaJr | 5′-GAT GTG TAT AAG AGA CAG-3′ | This study |
  hhaf | 5′-CAA ACC ATT AAC CAA AAC CG-3′ | This study |
  hhar | 5′-CGG AAT TTT ATC GTA GAG CTT-3′ | This study |
  fimAf | 5′-AAA CCG CGT TTA CTG G-3′ | This study |
  fimAr | 5′-GCA ACG GAG TTT GCT T-3′ | This study |
  16S rRNAf | 5′-GTG TTG TGA AAT GTT GGG T-3′ | This study |
  16S rRNAr | 5′-ACT AGC GAT TCC GAC TT-3′ | This study |