Skip to main content
Figure 6 | BMC Microbiology

Figure 6

From: S-Adenosyl-L-Methionine protects the probiotic yeast, Saccharomyces boulardii,from acid-induced cell death

Figure 6

AdoMet protects S. boulardii from ethanol and HCl-Induced cell death. S. boulardii cells (Florastor) were cultured in rich YPD media overnight and resuspended in fresh media and allowed to reach exponential phase. (A) They were then resuspended in fresh media, in fresh media containing 22% ethanol, in fresh media containing 1 mM AdoMet, or in in fresh media containing 22% ethanol and 1 mM AdoMet and allowed to grow at 30°C for the indicated times. Viability was measured as percentage colony forming units. (B) Next, S. boulardii cells were resuspended in water, water containing 75 mM HCl, water containing 75 mM HCl and 2 mM AdoMet, or water containing 2 mM AdoMet alone. They were allowed to grow at room temperature for 1.5 hours. Viability was measured as percentage colony forming units. (C) Exponential phase S. boulardii cells were resuspended in the indicated culture conditions and allowed to grow at room temperature for 1.5 hr. Intracellular ROS accumulation was detected with 5 μg/ml of dihydrorhodamine 123. (D) Activated caspase-like enzymatic activity was detected after treatment using a FLICA apoptosis detection kit according to the manufacturer’s specifications. At least three independent cultures were tested and compared. The differences were deemed statistically significant by the Student’s t-test (p<0.05)

Back to article page
\