Figure 4

S. boulardii undergoes programmed cell death in an acidic environment. S. boulardii (Florastor) was cultured in rich YPD media overnight and resuspended in fresh media and allowed to reach exponential phase. They were then resuspended in water or water containing 75 mM HCl and allowed to grow at room temperature for 1.5 hr. (A) Intracellular ROS accumulation was examined after treatment with 5 μg/ml of dihydrorhodamine 123. (B) Activated caspase-like activities were detected using a FLICA apoptosis detection kit according to the manufacturer’s specifications. At least three independent cultures were tested and compared. The differences were deemed statistically significant by the Student’s t-test (p<0.05)