Figure 3

S. boulardii cells are more viable in 50 mM HCl than their S. cerevisiae counterparts. S. boulardii (Florastor) and S. cerevisiae (W303α) were cultured in rich YPD media overnight and resuspended in fresh media and allowed to reach exponential phase. They were then resuspended in water or water containing 50 mM HCl and allowed to grow at room temperature for the indicated times, serially diluted onto YPD plates, and cultured at 30°C for 2 days. At least three independent cultures were tested and compared. The differences in viabilities were deemed statistically significant by the Student’s t-test (p<0.05)