|
Species
| |
PCR for detection
|
PCR for Sequencingd)
| | |
|---|
| |
Frequency of contaminationa)
|
tufgene (TaqMan PCR)b)
|
16S-23S ribosomal RNA intergenic region (nested PCR)c)
|
Match of new PCR primers
|
Strains
|
Sequence ID
|
|---|
|
Most common contaminant species
| | | | | | |
|
Mycoplasma fermentans
|
10%-20%
|
+
|
+
|
Match
|
human B cell lymphoma contaminants, 16054780
|
AY838558
|
|
Mycoplasma hyorhinis
|
10%-40%
|
+
|
+
|
Match
|
HUB-1
|
NC_014448.1
|
|
Mycoplasma orale
|
20%-30%
|
+
|
+
|
Partial Match
|
ATCC 23714D
|
gi|315440428
|
|
Mycoplasma arginini
|
20%-30%
|
No Data
|
+
|
Partial Match
|
G230
|
gi|290575476
|
|
Acholeplasma laidlawii
|
5%-20%
|
+
|
+
|
Match
|
PG-8A
|
CP000896
|
|
Mycoplasma hominis
|
10%-20%
|
+
|
+
|
Match
|
ATCC 23114
|
M57675
|
|
Other species
| | | | | | |
|
Mycoplasma arthritidis
|
No Data
|
+
|
No Data
|
Match
|
158L3-1
|
NC_011025.1
|
|
Mycoplasma bovis
|
No Data
|
+
|
No Data
|
Match
|
PG45
|
NC_014760.1
|
|
Mycoplasma buccale
|
No Data
|
+
|
No Data
|
No data
|
-
|
-
|
|
Mycoplasma faucium
|
No Data
|
+
|
No Data
|
No data
|
-
|
-
|
|
Mycoplasma gallisepticum
|
No Data
|
+
|
No Data
|
Match
|
PG31
|
X16462
|
|
Mycoplasma genitalium
|
No Data
|
+
|
+
|
Match
|
ATCC33530
|
X16463
|
|
Mycoplasma hyopneumoniae
|
No Data
|
+
|
No Data
|
Match
|
7448
|
NC_007332.1
|
|
Mycoplasma penetrans
|
No Data
|
+
|
No Data
|
Match
|
HF-2
|
NC_004432.1
|
|
Mycoplasma pneumoniae
|
No Data
|
+
|
+
|
Match
|
FH
|
X55768
|
|
Mycoplasma primatum
|
No Data
|
+
|
No Data
|
No data
|
-
|
-
|
|
Mycoplasma salivarium
|
No Data
|
+
|
+
|
Partial Match
|
ATCC 23064D
|
gi|313575713
|
|
Ureaplasma parvum
|
No Data
|
+
|
No Data
|
Match
|
ATCC 33697
|
AF270770
|
|
Mycoplasma zalophi
|
No Data
|
No Data
|
No Data
|
Match
|
CSL 4296
|
gi|148536300
|
|
Mycoplasma mycoides
|
No Data
|
No Data
|
No Data
|
Match
|
PG1
|
gi|126252003
|
|
Mycoplasma capricolum
|
No Data
|
No Data
|
No Data
|
Match
|
ATCC 27343
|
gi|83319253
|
|
Mycoplasma agalactiae
|
No Data
|
No Data
|
No Data
|
Match
|
PG2
|
gi|148291314
|
|
Mycoplasma pyrum
|
No Data
|
No Data
|
+
|
No data
|
-
|
-
|
- a) Upper 6 species of mycoplasmas are the most common contaminants of cell cultures [11, 12].
- b) This broad-range TaqMan PCR can detect many species of mycoplasmas [22].
- c) This nested PCR is highly sensitive, and it is used to check for mycoplasma contamination in the Cell Bank of BioResource Centre, Riken Tsukuba Institute, Tsukuba, Ibaraki, Japan [21].
- d) PCR assay for sequencing of mycoplasmas designed in this study. Partial Match means that 2 or 3 of the total of 4 nested-PCR primers match to available regions of the tuf gene on the public database.
