Table 2 Major mycoplasmas, and their detection and sequencing methods in this study
Species | PCR for detection | PCR for Sequencingd) | ||||
|---|---|---|---|---|---|---|
Frequency of contaminationa) | tufgene (TaqMan PCR)b) | 16S-23S ribosomal RNA intergenic region (nested PCR)c) | Match of new PCR primers | Strains | Sequence ID | |
Most common contaminant species | ||||||
Mycoplasma fermentans | 10%-20% | + | + | Match | human B cell lymphoma contaminants, 16054780 | AY838558 |
Mycoplasma hyorhinis | 10%-40% | + | + | Match | HUB-1 | NC_014448.1 |
Mycoplasma orale | 20%-30% | + | + | Partial Match | ATCC 23714D | gi|315440428 |
Mycoplasma arginini | 20%-30% | No Data | + | Partial Match | G230 | gi|290575476 |
Acholeplasma laidlawii | 5%-20% | + | + | Match | PG-8A | CP000896 |
Mycoplasma hominis | 10%-20% | + | + | Match | ATCC 23114 | M57675 |
Other species | ||||||
Mycoplasma arthritidis | No Data | + | No Data | Match | 158L3-1 | NC_011025.1 |
Mycoplasma bovis | No Data | + | No Data | Match | PG45 | NC_014760.1 |
Mycoplasma buccale | No Data | + | No Data | No data | - | - |
Mycoplasma faucium | No Data | + | No Data | No data | - | - |
Mycoplasma gallisepticum | No Data | + | No Data | Match | PG31 | X16462 |
Mycoplasma genitalium | No Data | + | + | Match | ATCC33530 | X16463 |
Mycoplasma hyopneumoniae | No Data | + | No Data | Match | 7448 | NC_007332.1 |
Mycoplasma penetrans | No Data | + | No Data | Match | HF-2 | NC_004432.1 |
Mycoplasma pneumoniae | No Data | + | + | Match | FH | X55768 |
Mycoplasma primatum | No Data | + | No Data | No data | - | - |
Mycoplasma salivarium | No Data | + | + | Partial Match | ATCC 23064D | gi|313575713 |
Ureaplasma parvum | No Data | + | No Data | Match | ATCC 33697 | AF270770 |
Mycoplasma zalophi | No Data | No Data | No Data | Match | CSL 4296 | gi|148536300 |
Mycoplasma mycoides | No Data | No Data | No Data | Match | PG1 | gi|126252003 |
Mycoplasma capricolum | No Data | No Data | No Data | Match | ATCC 27343 | gi|83319253 |
Mycoplasma agalactiae | No Data | No Data | No Data | Match | PG2 | gi|148291314 |
Mycoplasma pyrum | No Data | No Data | + | No data | - | - |