Figure 1From: Deletion of pic results in decreased virulence for a clinical isolate of Shigella flexneri2a from ChinaAmplicons from SF51 int , orf30 , sigA and pic . Specific amplicons for (A) int; (B) orf30; (C) sigA; and (D) pic. The target genes int, orf30 and sigA were amplified from SF51 DNA but pic was not. All four target genes were amplified from the SF301 positive control. The sequence of all PCR products was confirmed by nucleic acid sequencing.Back to article page