From: Release of extracellular ATP by bacteria during growth
Oligonucleotides | Used for | Sequence |
---|---|---|
cyoA5KO | Mutagenesis of cyoA | 5′-ctcaggaaatacaataaaagtttgggatggttgtcattaattgcaggcactgcattactcagtggct gtaattctgcgctgctggatcccgtgtaggctggagctgcttc-3′ |
cyoA3KO | Mutagenesis of cyoA | 5′-caacccttggagttggcggattccgcgtggctcatgtccataccttccattccttcatgcgagctgtgc tcaccttcaggttgggtcatgcatatgaatatcctccttag-3′ |
cyoB5KO | Mutagenesis of cyoB | 5′-ataataagcaatcgttgcctgcgattaccctcgcagctattggggttgtctacggtgatattggt accagcccgctttatacgcttcgtgaatgtttgtcgtgtaggctggagctgcttc-3′ |
cyoB3KO | Mutagenesis of cyoB | 5′-aaattatcactggatgcagtaccgttccatgaacctatcgtcatggtaacgatcgctgcaattatcgtc gggggactggcgatactggcagtgtaggctggagctgcttc -3′ |
cyoC5KO | Mutagenesis of cyoC/D mutation | 5′-tgcattctgttctctattctgtttgctacctatgccgttctggtgaacggcaccgctggcggcccgacaggt aaggacattttcgaactggtgtaggctggagctgcttc -3′ |
cyoD3KO | Mutagenesis of cyoC/D mutation | 5′-tgtagttgaggttccacataatccagatggagcccacaaccaggatggcgatgatcagcacggtaaag atgaaggccgtcatgttccagcatatgaatatcctccttag -3′ |
cyoA5 | Confirmation of cyoA mutation | 5′-atcatgtttacagtaatgta-3′ |
cyoA3 | Confirmation of cyoA mutation | 5′-tccgaacatcttatcttcct -3′ |
cyoB5 | Confirmation of cyoB mutation | 5′-aggaagataagatgttcgga-3′ |
cyoB3 | Confirmation of cyoB mutation | 5′-tcgcgtgcgttaaagtatca -3′ |
cyoCD5 | Confirmation of cyoC/D mutation | 5′-tgatactttaacgcacgcga -3′ |
cyoCD3 | Confirmation of cyoC/D mutation | 5′-tgcaggtattgcttaaacat-3′ |
K1 | KanR primer for confirmation of mutation | 5′-cagtcatagccgaatagcct-3′ |
K2 | KanR primer for confirmation of mutation | 5′-cggtgccctgaatgaactgca-3′ |