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Table 2 Diagnostic sensitivity of the PCR

From: A novel, multiplex, real-time PCR–based approach for the detection of the commonly occurring pathogenic fungi and bacteria

Microbial strains

No. (%) of positive PCRs*

Gram positive (G+)

50 copies

10 copies

5 copies

2 copies

1 copy

Enterococcus faecalis

8 (100)

8 (100)

5 (62.5)

2 (25)

0 (0)

Staphylococcus aureus

8 (100)

8 (100)

7 (87.5)

3 (37.5)

0 (0)

Streptococcus pyogenes

8 (100)

8 (100)

5 (62.5)

5 (62.5)

0 (0)

Gram negative (G-)

     

Enterobacter aerogenes

8 (100)

8 (100)

5 (62.5)

2 (25)

0 (0)

Escherichia coli

8 (100)

8 (100)

6 (75)

1 (12.5)

0 (0)

Haemphilus influenzae

8 (100)

7 (87.5)

4 (50)

0 (0)

0 (0)

Fungi

     

Candida albicans

8 (100)

8 (100)

5 (62.5)

0 (0)

0 (0)

Candida tropicalis

8 (100)

8 (100)

6 (75)

1 (12.5)

0 (0)

  1. *Out of 8 samples.
  2. Three Gram positive, three Gram negative and two fungal strains were used for the infection of healthy donor bloods. All the experiments were carried out eight times using 5 dilutions of the pathogens.