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Table 2 Diagnostic sensitivity of the PCR

From: A novel, multiplex, real-time PCR–based approach for the detection of the commonly occurring pathogenic fungi and bacteria

Microbial strains No. (%) of positive PCRs*
Gram positive (G+) 50 copies 10 copies 5 copies 2 copies 1 copy
Enterococcus faecalis 8 (100) 8 (100) 5 (62.5) 2 (25) 0 (0)
Staphylococcus aureus 8 (100) 8 (100) 7 (87.5) 3 (37.5) 0 (0)
Streptococcus pyogenes 8 (100) 8 (100) 5 (62.5) 5 (62.5) 0 (0)
Gram negative (G-)      
Enterobacter aerogenes 8 (100) 8 (100) 5 (62.5) 2 (25) 0 (0)
Escherichia coli 8 (100) 8 (100) 6 (75) 1 (12.5) 0 (0)
Haemphilus influenzae 8 (100) 7 (87.5) 4 (50) 0 (0) 0 (0)
Fungi      
Candida albicans 8 (100) 8 (100) 5 (62.5) 0 (0) 0 (0)
Candida tropicalis 8 (100) 8 (100) 6 (75) 1 (12.5) 0 (0)
  1. *Out of 8 samples.
  2. Three Gram positive, three Gram negative and two fungal strains were used for the infection of healthy donor bloods. All the experiments were carried out eight times using 5 dilutions of the pathogens.