Table 1 Range and levels of the independent variables lysine (Lys) and alpha-aminoadipic acid (AAA), in coded and original units, according to the two-factor, three-level central-composite-based, face-centered, experimental design (CCF); the response variable is cephamycin C concentration (CephC) obtained at 72-hour cultivation
Run | Independent variables | Response | ||||
|---|---|---|---|---|---|---|
Coded units | Original units (g l-1) | CephC (mg l-1) | ||||
x Lys | x AAA | x Lys | x AAA | Measured* | Predicted | |
1 | -1 | -1 | 0.9 | 0 | 25.0 ± 8.2 | 15.5 |
2 | 0 | -1 | 3.2 | 0 | 45.0 ± 9.6 | 52.7 |
3 | +1 | -1 | 5.5 | 0 | 55.0 ± 5.9 | 56.7 |
4 | -1 | 0 | 0.9 | 0.32 | 44.1 ± 0.9 | 57.8 |
5 | 0 | 0 | 3.2 | 0.32 | 105.8 ± 6.6 | 100.5 |
6 | +1 | 0 | 5.5 | 0.32 | 118.5 ± 6.4 | 110.0 |
7 | 0 | +1 | 3.2 | 0.64 | 112.4 ± 0.0 | 110.6 |
8 | 0 | +1 | 3.2 | 0.64 | 102.8 ± 0.0 | 110.6 |
9 | 0 | +1 | 3.2 | 0.64 | 117.8 ± 0.0 | 110.6 |
10 | 0 | +1 | 3.2 | 0.64 | 112.0 ± 0.0 | 110.6 |
11 | -1 | +1 | 0.9 | 0.64 | 66.7 ± 7.7 | 62.4 |
12 | +1 | +1 | 5.5 | 0.64 | 118.8 ± 9.6 | 125.6 |