Table 1 Sequence of PCR primers and molecular beacon probes
PCR primers/Probes/Oligos | Sequence* | Length | Tm (°C) | Size of PCR amplicon | Fluorophore/Quencher |
|---|---|---|---|---|---|
RecF primer | 5’ GTG GAT CTA TTG TAT TAG ATG AGG CTC TCG 3’ | 30 | 66.1 | 222 bp |  |
RecR primer | 5’ GCC AAA GTT CTG CAA CAT TAA CAC CTA AAG 3’ | 30 | 67.3 |  | |
RecF3 primer | 5’ GCA AGA GTT CAA ATA GAA AA 3’ | 20 | 53.7 | 287 bp |  |
RecR3 primer | 5’ AAA GCT TTT GCA TAA ACA G 3’ | 19 | 54.7 |  | |
RecA3 probe | 5’ CTG GCG GAT ATC CTA GGG GG CGC CAG 3’ | 26 | 67.9 |  | FAM/ BHQ-1 |
5BmicrotiTPK primer | 5’ AAT ATT GTT GAA TGG GGA TAT TTG TG 3’ | 26 | 64.2 | 600 bp |  |
3BmicrotiTPK primer | 5’ AAT AAT ATA GCT TTT CCA AAA TAT AAC TGA C 3’ | 31 | 60.2 |  | |
5BmTPK primer | 5’ TGA GAG GAA CGA CCA TAG C 3’ | 19 | 61.4 | 141 bp |  |
3BmTPK primer | 5’ CCA TCA GGT AAA TCA CAC GAA A 3’ | 22 | 61.6 |  | |
BmTPK probe | 5’ CGC GTC GGT GTT GTT GAC CAG CGG CCG CG GAC GCG 3’ | 35 | 61.5 |  | CAL Fluor Orange 560/ BHQ-1 |
5ApAPH1387 primer | 5’ ATG TAT GGT ATA GAT ATA GAG CTA AGT GA 3’ | 29 | 57.8 | 1737 bp |  |
3ApAPH1387 primer | 5’ CTA ATA ACT TAG AAC ATC TTC ATC GTC AG 3’ | 29 | 62.2 |  | |
5Aphagocyt primer | 5’ ATG GCT ACT ACG AAG GAT 3’ | 18 | 57.9 | 152 bp |  |
3Aphagocyt primer | 5’ CGA AGC AAC ATC TCT ACA T 3’ | 19 | 58.0 |  | |
Aph1387 probe | 5’ CGG TGC GAC AAA GAT GCC AGC ACT AAT GCG GCA CCG 3’ | 36 | 61.9 |  | CAL Fluor Red 610/ BHQ-2 |
5ACTA1 primer | 5’ AGA GCA AGA GAG GTA TCC 3’ | 18 | 58.0 | 104 bp |  |
3ACTA1 primer | 5’ CTC GTT GTA GAA GGT GTG 3’ | 18 | 57.7 |  | |
ACTA1 probe | 5’ CGC TGC CCT ATC GAG CAC GGC ATC ATC AC GCA GCG 3’ | 35 | 62.4 |  | Quasar 670/ BHQ-2 |
RecA3MB-com oligo | 5’ ttG CGC CCC CTA GGA TAT CCG Ctt 3’ | 24 | 67.9 |  |  |
TPKMB-com oligo | 5’ tt tCG CGG CCG CTG GTC AAC AAC ACC ttt 3’ | 29 | 61.5 |  |  |
AphMB-com oligo | 5’ ttt CGC ATT AGT GCT GGC ATC TTT GTC ttt 3’ | 30 | 61.9 |  |  |
ActinMB-com oligo | 5’ tt tGT GAT GAT GCC GTG CTC GAT AGG ttt 3’ | 29 | 62.4 |  |  |