Skip to main content

Table 2 The purification table of C-His-Rv2135c from 1 liter culture of E. coli BL21(DE3)-35c

From: Functional characterization of two members of histidine phosphatase superfamily in Mycobacterium tuberculosis

Step Protein (mg) Activity (U) Specific activity (U/mg) Purification (fold) Yield (%)
Clarified extract 464 18.60 0.04 1.00 100
Eluted fractions from IMAC 50.40 11.60 0.23 5.60 62.40
  1. The activities are reported using pNPP as substrate at pH 5.8.