Table 1 The purification table of C-His-Rv0489c from 1 liter culture of E. coli BL21(DE3)-89c
Step | Protein (mg) | Activity (U) | Specific activity (U/mg) | Purification (fold) | Yield (%) |
|---|---|---|---|---|---|
Clarified extract | 166.50 | 3696 | 22.20 | 1.00 | 100 |
Eluted fractions from IMAC | 26.50 | 1432 | 54.00 | 2.40 | 38.70 |