Table 1 Bacterial strains and plasmids
Strains and plasmids | Relevant genotypes and derivation | Source and reference |
|---|---|---|
B. japonicum | ||
USDA110 | 24 | |
E. coli | ||
DH5a | supE44, DlacU169, hsdR17, recA1, endA1, gyrA96, thi-1, relA1 | Laboratory stocks |
BL21 (DE3) | F-ompT hsdSb (rb-mb-) gal dcm (DE3) | Laboratory stocks |
Plasmids | ||
pET-28b | Protein expression vector, kanamycin resistant | Takara Bio |
pETPhaP1 | pET28b carrying phaP1 | This work |
pETPhaP2 | pET28b carrying phaP2 | This work |
pETPhaP3 | pET28b carrying phaP3 | This work |
pETPhaR | pET28b carrying phaR | This work |
pColdII | Protein expression vector, ampicillin resistant | Takara Bio |
pColdPhaP4 | pColdII carrying phaP4 | This work |