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Table 2 Universal bacterial primers and group-specific primers (based on 16S rRNA) and fungal primers (based on 18S rRNA) used for PCR amplification of L. sidoides stem and leaf DNA for DGGE evaluation

From: Does the essential oil of Lippia sidoidesCham. (pepper-rosmarin) affect its endophytic microbial community?

Communities

Primers

Reference

        Sequences a

Total bacteria

*U968/L1401

[26]

*5â€ČACCGCGAAGAACCTTAC3â€Č/

5â€ČGCGTGTGTACAAGACCC3â€Č

Total bacteria

799F/1492R

[29]

5â€ČAACMGGATTAGATACCCKG3â€Č/

*U968/L1401

[26]

5â€ČTACGGYTACCTTGTTACGACT3â€Č

Alphaproteobacteria

F203α/L1401

[30]

5â€ČCCGCATACGCCCTACGGGGGAAAGATTTAT3â€Č

*U968/L1401

[26]

Betaproteobacteria

F948ÎČ/L1401

[30]

5â€ČCGCACAAGCGGTGGATGA3â€Č

*U968/L1401

[26]

Actinobacteria

F243/L1401

[27]

5â€Č GGATGAGCCCGCGGCCTA 3â€Č

*U968/L1401

[26]

Fungi

EF4/ITS4

[28]

5â€ČGGAAGGGRTGTATTTATTAG3â€Č/

*ITS1f/ITS2

[24]

5â€Č TCCTCCGCTTATTGATATGC3â€Č

[25]

*5â€ČCTTGGTCATTTAGAGGAAGTAA3â€Č/

  

[24]

5â€ČGCTGCGTTCTTCATCGATGC3â€Č

  1. a The sequences correspond to the primers in bold.
  2. * Primer with a 40 bp GC-clamp (5â€Č- CGCCCGCCGCGCGCGGCGGGCGGGGCGGGGGCACGGGGGG –3â€Č) attached.