Table 2 Primers used in this study
Primer name | Oligonucleotide (5′-3′)a | Application |
|---|---|---|
up-airSR-f | CCGgaattcTACATCTTGTGCCTTAG | airSR deletion |
up-airSR-r | ATTTGAGatcgatAATGTTCAG | airSR deletion |
down-airSR-f | CGATTTAAGTggtaccGTTGCATGATGTG | airSR deletion |
down-airSR-r | CGCggatccCCTTAAGTTGTTGGAA | airSR deletion |
Em-f | CGGatcgatGATACAAATTCCCCGTAGGC | airSR deletion |
Em-r | CGGggtaccGAAATAGATTTAAAAATTTCGC | airSR deletion |
c-airSR-f | CGCggatccATCGTCGCCAGTATG | ΔairS complementation |
c-airSR-r | CCGgaattcTGAAGCGAAAGTAAATG | ΔairS complementation |
e-airR-f | GGAATTCcatatgAACAAAGTAATATT | expression of AirR |
e-airR-r | CCGctcgagAATCAACTTATTTTCCA | expression of AirR |
e-airS-f | GGGAATTCcatatgATGGAACAAAGGACGCGACTAG | expression of AirS |
e-airS-r | CCGctcgagCTATTTTATAGGAATTGTGAATTG | expression of AirS |
RTQ-cap5B-f | GCTTATTGGTTACTTCTGA | real-time RT PCR |
RTQ-cap5B-r | GTTGGCTTACGCATATC | real-time RT PCR |
RTQ-cap5D-f | ATATGCCAGTGTGAGTGA | real-time RT PCR |
RTQ-cap5D-r | CGGTCTATTGCCTGTAAC | real-time RT PCR |
RTQ-lytM-f | CATTCGTAGATGCTCAAGGA | real-time RT PCR |
RTQ-lytM-r | CTCGCTGTGTAGTCATTGT | real-time RT PCR |
RTQ-640-f | TGATGGGACAGGAGT | real-time RT PCR |
RTQ-640-r | TATTGTGCCGCTTCT | real-time RT PCR |
RTQ-953-f | GTCATTGAGCACGATTTATT | real-time RT PCR |
RTQ-953-r | TCTGGGCGGCTGTAA | real-time RT PCR |
RTQ-pbp1-f | AGTCAGCGACCAACATT | real-time RT PCR |
RTQ-pbp1-r | AAGCACCTTCTTGAATAGC | real-time RT PCR |
RTQ-murD-f | TTCAGGAATAGAGCATAGA | real-time RT PCR |
RTQ-murD-r | AACCACCACATAACCAA | real-time RT PCR |
RTQ-1148-f | GCCGAAGTGACATAC | real-time RT PCR |
RTQ-1148-r | AAGCACCGACTGATA | real-time RT PCR |
RTQ-ddl-f | TAGGGTCAAGTGTAGGT | real-time RT PCR |
RTQ-ddl-r | GTCGCTTCAGGATAG | real-time RT PCR |
RTQ-pta-f | AAAGCGCCAGGTGCTAAATTAC | real-time RT PCR |
RTQ-pta-r | CTGGACCAACTGCATCATATCC | real-time RT PCR |
p-cap5A-f | TCATCTAACTCACCTGAAATTACAAAA | EMSA |
p-cap5A-r | TTTCCATTATTTACCTCCCTTAAAAA | EMSA |
p-ddl-f | CAAACTCCTTTTATACTC | EMSA |
p-ddl-r | GTCATTTCGTTTTCCT | EMSA |
p-pbp1-f | GATTCAATAGAACAAGCGATT | EMSA |
p-pbp1-r | AGCTACACGTAATTTCGCGCTT | EMSA |
p-lytM-f | GAATCGCGAACATGGACGAA | EMSA |
p-lytM-r | GCAATCGCTGCTGCTGTTAA | EMSA |